| 甜樱桃品种SSR指纹检索系统的开发及遗传多样性分析 |
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| 引用本文： | 张琪静,张新忠,代红艳,谷大军,闫桂军,李春敏,张志宏.甜樱桃品种SSR指纹检索系统的开发及遗传多样性分析[J].园艺学报,2008,35(3):329-336. |
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| 作者姓名： | 张琪静 张新忠 代红艳 谷大军 闫桂军 李春敏 张志宏 |
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| 作者单位： | (沈阳农业大学园艺学院, 沈阳110161; 辽宁省果树科学研究所, 辽宁熊岳115214; 河北省农林科学院昌黎果树研究所, 河北昌黎066600; 西澳大学, 珀斯6009, 澳大利亚) |
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| 基金项目： | 辽宁省重点实验室专题项目 |
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| 摘 要： | 用SSR技术开发了甜樱桃( Prunus avium ) 指纹检索系统并进行遗传多样性分析。18对樱桃、桃和杏的引物在19份甜樱桃及2份草原樱桃( P. fruticosa) 品种中共扩增出83个等位位点, 每个 SSR位点的等位位点数2 ～8 个, 平均416 个, 多态性信息量( PIC) 变化范围为0.38 ～0.80, 平均为 0.64。7个甜樱桃品种具有特殊位点或特殊带型。利用UDP98-414、UDP98-406、UDP96-001及PMS40等4 对引物开发的指纹检索系统, 可以区分18个甜樱桃品种。根据遗传距离进行聚类分析, 19个甜樱桃品种 分成2组, 甜樱桃品种间的聚类结果基本反映了供试材料之间的亲缘关系。
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| 关 键 词： | 甜樱桃 SSR 遗传多样性 品种鉴定 指纹检索系统 |
| 收稿时间： | 2007-09-01 |
Development of Fingerprinting Key and Analysis of Genetic Diversity with SSR Marker for Sweet Cherry Cultivars |
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| ZHANG Qi-jing,ZHANG Xin-zhong,DAI Hong-yan,GU Da-jun,YAN Gui-jun,LI Chun-min,ZHANG Zhi-hong.Development of Fingerprinting Key and Analysis of Genetic Diversity with SSR Marker for Sweet Cherry Cultivars[J].Acta Horticulturae Sinica,2008,35(3):329-336. |
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| Authors: | ZHANG Qi-jing ZHANG Xin-zhong DAI Hong-yan GU Da-jun YAN Gui-jun LI Chun-min ZHANG Zhi-hong |
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| Institution: | (;1.College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China;2.Liaoning Institute of Pomology, Xiongyue, Liaoning 115214, China;3.Changli Fruit Research Institute, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China;4. The University of Western Australia, Perth 6009, Australia) |
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| Abstract: | SSR technique was used to develop fingerprinting key and study the genetic diversity on sweet cherry. Eighteen pairs of primers cloned from cherry, peach and apricot amplified a total of 83 alleles among the 19 cultivars of sweet cherry ( Prunus avium ) and 2 cultivars of ground cherry ( P. fruticosa). The number of alleles amplified per primer pair ranged from 2 to 8, with a mean of 416. The polymorphic information content values ( PIC) ranged from 0.38 to 0.80, with a mean of 0.64. Seven sweet cherry cultivars showed unique bands or banding patterns. A fingerp rinting key developed by 4 pairs of p rimers: UDP98-414, UDP98-406, UDP96-001 and PMS40 could discriminate 18 sweet cherry cultivars. UPGMA cluster analysis of genetic distance separated the 19 cultivars of sweet cherry into two group s. The phylogeny of sweet cherry cultivars revealed the closed relationships among cultivars tested. |
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| Keywords: | sweet cherry SSR genetic diversity cultivar identification fingerprinting key |
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