| 乳杆菌的分离及鉴定 |
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| 引用本文： | 崔艳伟,孟祥晨.乳杆菌的分离及鉴定[J].东北农业大学学报,2008,39(6). |
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| 作者姓名： | 崔艳伟 孟祥晨 |
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| 作者单位： | 东北农业大学乳品科学教育部重点实验室,哈尔滨,150030 |
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| 摘 要： | 从健康学龄儿童的新鲜粪便样品中分离得到6株乳杆菌,并对其进行属和种的鉴定。首先通过形态学观察及H2O2酶、糖发酵等生理生化试验对所分得菌株进行初步鉴定,然后利用16S rDNA序列同源性分析方法,对所分得菌株进行16S rDNA基因扩增与测序,测序结果与GenBank中该属内菌株的16S rDNA基因序列进行同源性分析,从而准确鉴定所分得菌株,并申请各菌株的GenBank登录号。经鉴定,其中1株为卷曲乳杆菌,2株为植物乳杆菌,1株为发酵乳杆菌,1株为黏液乳杆菌,1株为唾液乳杆菌。
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| 关 键 词： | 乳杆菌 粪便 分离 鉴定 |
Isolation and identification of several Lactobacilli |
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| CUI Yanwei,MENG Xiangchen.Isolation and identification of several Lactobacilli[J].Journal of Northeast Agricultural University,2008,39(6). |
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| Authors: | CUI Yanwei MENG Xiangchen |
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| Abstract: | Six Lactobacilli strains were isolated from fresh feces samples of healthy school age children and identified to the level of genus and species.Firstly,these strains were identified by the phenotypic tests,sugar fermentation and catalase tests.Then,the method of 16S rDNA sequence homology analysis was used,the genes of 16S rDNA of these strains were amplified and sequenced.Finally,the sequence results about these strains were compared with other 16S rDNA sequences of Lactobacilli in GenBank,thus these strains could be differentiated exactly.And applying for GenBank accession numbers of these isolates,1 strain was Lactobacillus crispatus,2 strains were Lactobacillus plantarum,1 strain was Lactobacillus feimentum,1 strain was Lactobaci-llus mucosae and 1 strain was Lactobacillus salivarius. |
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| Keywords: | Lactobacilli feces isolation identification |
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