| EuFPS基因表达载体构建及对杜仲遗传转化的研究 |
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| 引用本文： | 赵丹,赵德刚,李岩.EuFPS基因表达载体构建及对杜仲遗传转化的研究[J].广西农业生物科学,2009(1). |
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| 作者姓名： | 赵丹 赵德刚 李岩 |
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| 作者单位： | 贵州大学贵州省农业生物工程重点实验室;贵州大学生命科学学院基因工程实验室;教育部绿色农药与农业生物工程重点实验室; |
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| 基金项目： | 国家自然科学基金项目(30660146);;贵州省优秀科技、教育人才省长基金(黔省专合字(2005)351号)资助 |
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| 摘 要： | 本实验用EcoRⅠ和BamHⅠ双酶切植物表达载体pSH737和含有目的基因的pUC-FPS,定向连接得到重组质粒pSH-FPS,将其导入农杆菌EHA105。采用农杆菌介导法对杜仲进行遗传转化,研究了卡那霉素(kanamycin,Km)浓度、预培养时间、菌液浓度及侵染时间、乙酰丁香酮(acetosyringone,AS)浓度、共培养时间等对杜仲遗传转化效率的影响。结果表明,选择无菌苗苗龄15d的杜仲下胚轴,卡那霉素浓度50mg/L,农杆菌浓度OD600值0.3~0.6,侵染时间8min,侵染时菌体重悬液中添加50μmol/L乙酰丁香酮,共培养时间3d,抗性芽的获得率最高。对再生植株进行GUS检测发现有45%的植株呈阳性。
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| 关 键 词： | 杜仲 FPS基因 植物表达载体 遗传转化 |
Studies on Construction of EuFPS Gene Plant Expression Vector and Agrobacterium-Mediated Genetic Transformation of Eucommia ulmoides Oliv. |
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| Zhao Dan , Zhao Degang , Li Yan , Guizhou Key Laboratory of Agro-Bioengineering,Guiyang, The Laboratory of Gene engineering,College of Life Sciences,Guizhou University, KeyLaboratoryofGreen Pesticide , Agricultural Biological Engineering MinistryofEducation.Studies on Construction of EuFPS Gene Plant Expression Vector and Agrobacterium-Mediated Genetic Transformation of Eucommia ulmoides Oliv.[J].Journal of Guangxi Agricultural and Biological Science,2009(1). |
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| Authors: | Zhao Dan Zhao Degang Li Yan Guizhou Key Laboratory of Agro-Bioengineering Guiyang The Laboratory of Gene engineering College of Life Sciences Guizhou University KeyLaboratoryofGreen Pesticide Agricultural Biological Engineering MinistryofEducation |
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| Institution: | Zhao Dan 1,2 Zhao Degang 1,2,3 Li Yan 1,2 1 Guizhou Key Laboratory of Agro-Bioengineering,Guiyang,550025,2 The Laboratory of Gene engineering,College of Life Sciences,Guizhou University,3 KeyLaboratoryofGreen Pesticide , Agricultural Biological Engineering MinistryofEducation |
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| Abstract: | |
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| Keywords: | Eucommia ulmoides Oliv FPS gene Plant expression vector Genetic Transformation |
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