| Wistar大鼠超数排卵及采集不同时期胚胎的初步研究 |
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| 引用本文： | 张丽,谭竹钧,高珊,陈炜,刘牧.Wistar大鼠超数排卵及采集不同时期胚胎的初步研究[J].黑龙江畜牧兽医,2012(13):11-14. |
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| 作者姓名： | 张丽 谭竹钧 高珊 陈炜 刘牧 |
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| 作者单位： | 广东工业大学轻工化工学院;中国医学科学院医学实验动物研究所 |
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| 基金项目： | 卫生部“实验动物和人类疾病动物模型资源扩展”项目(20080236) |
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| 摘 要： | 为了研究获取Wistar大鼠不同发育阶段胚胎所需超排试剂的最佳剂量以及不同发育期胚胎的采集方法,试验采用10 IU/只、20 IU/只和30 IU/只3种剂量的孕马血清促性腺激素-人绒毛膜促性腺激素(PMSG-HCG)对8～9周龄的Wistar大鼠进行超排处理。结果表明:采集2-细胞期胚胎时,分别获得胚胎(12.1±4.6)枚、(23.1±10.2)枚和(24.9±8.2)枚,其中激素剂量为30 IU/只时采集2-细胞期胚胎超排效果最好;在采集桑葚胚期胚胎时,分别可获得胚胎(10.3±5.0)枚、(23.7±17.2)枚和(17.2±5.9)枚,其中激素剂量为20 IU/只的超排效果最好。
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| 关 键 词： | 大鼠 Wistar 超数排卵 胚胎 |
A preliminary study on superovulation and the embryos of Wistar rats at different developmental stages |
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| ZHANG Li,TAN Zhu-jun,GAO Shan,CHEN Wei,LIU Mu.A preliminary study on superovulation and the embryos of Wistar rats at different developmental stages[J].Heilongjiang Animal Science And veterinary Medicine,2012(13):11-14. |
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| Authors: | ZHANG Li TAN Zhu-jun GAO Shan CHEN Wei LIU Mu |
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| Institution: | 1.College of Light Industry and Chemical Engineering,Guangdong University of Technology,Guangzhou 510006,China; 2.Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences,Beijing 100021,China) |
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| Abstract: | To obtain the methods for embryo collection and the optimal dose of the reagents for superovulation to be used in Wistar rats at different developmental stages,eight to nine-week-old Wistar rats were superovulated with 10 IU,20 IU and 30 IU of pregnant mare serum gonadotropin-human chorionic gonadotropin(PMSG-HCG),respectively.The results showed that the number of two-cell embryos obtained from the three groups were 12.1±4.6,23.1±10.2 and 24.9±8.2 respectively,in which the superovulation with 30 IU was considered to be the best one.The numbers of morula obtained from the three groups were 10.3±5.0,23.7±17.2 and 17.2±5.9 respectively,in which the superovulation with 20 IU was considered to be the best one. |
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| Keywords: | rat Wistar superovulation embryo |
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