| 用马铃薯块茎全蛋白聚丙烯酰胺凝胶电泳技术鉴定品种的真实性和纯度 |
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| 引用本文： | 吴凌娟,董传民,张亚奎,温福君.用马铃薯块茎全蛋白聚丙烯酰胺凝胶电泳技术鉴定品种的真实性和纯度[J].中国马铃薯,1997(2). |
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| 作者姓名： | 吴凌娟 董传民 张亚奎 温福君 |
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| 作者单位： | 大兴安岭地区马铃薯科研中心 |
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| 摘 要： | 试用Ｓｔｅｇｅｍａｎｎ报道的电泳方法，用一小块薯肉提取全蛋白，对３０个马铃薯品种进行电泳分析。结果看到，所有品种的全蛋白电泳图谱都有差异，且品种内个体间的图谱表现一致。这表明品种的全蛋白电泳图谱与遗传因子有关，是鉴定品种的可靠特性。因此，块茎全蛋白电泳技术可用于马铃薯品种真实性鉴定和纯度检验。本法具有分辨力高、简便、快速等特点
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| 关 键 词： | 马铃薯品种，块茎全蛋白电泳技术 |
TESTING TRUENESS AND PURITY OF POTATO VARIETY BY TUBER TOTAL PROTEIN POLYACRYLAMIDE GEL ELECTROPHORESIS TECHNIQUE |
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| Wu Lingjuan,Dong Chuanmin,Zhang Yakui and Wen Fujun.TESTING TRUENESS AND PURITY OF POTATO VARIETY BY TUBER TOTAL PROTEIN POLYACRYLAMIDE GEL ELECTROPHORESIS TECHNIQUE[J].Chinese Potato,1997(2). |
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| Authors: | Wu Lingjuan Dong Chuanmin Zhang Yakui and Wen Fujun |
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| Institution: | Daxinganling Potato Research Center 165000 |
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| Abstract: | Total protein was extracted from tubers of each of 30 varieties and electrophoresis was carried out, using modified Stegemann's method. The results indicated that the total protein electrophoresis diagrams were different for these varieties tested, but the diagram was the same for different individual of a variety, suggesting that the diagram is related to hereditary factors of a variety. Therefore, this method can be used to test trueness and purity of potato variety. The method used in this experiment is accurate, simple and rapid, and is suitable for ordinary laboratory. |
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| Keywords: | potato variety electrophoresis |
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