| 宠物源大肠埃希菌的分离鉴定和耐药性研究 |
| |
| 引用本文： | 刘果,纪雪,孙洋,刘哲,祝令伟,刘军,周伟,郭学军,冯书章.宠物源大肠埃希菌的分离鉴定和耐药性研究[J].动物医学进展,2016(2):120-124. |
| |
| 作者姓名： | 刘果 纪雪 孙洋 刘哲 祝令伟 刘军 周伟 郭学军 冯书章 |
| |
| 作者单位： | 军事医学科学院军事兽医研究所吉林省人兽共患病预防与控制重点实验室,吉林长春,130122 |
| |
| 基金项目： | 国家科技部传染病重大专项，863项目，吉林省科技计划项目 |
| |
| 摘 要： | 为调查长春地区宠物源大肠埃希菌耐药流行情况,从2家宠物医院采集135份宠物肛拭子样品,分离和鉴定大肠埃希菌并进行多重PCR分群。测定大肠埃希菌分离株对19种抗菌药物的耐药性,并鉴定超广谱β-内酰胺酶(ESBLs)表型。共分离鉴定获得95株宠物源大肠埃希菌,它们对氨苄西林和哌拉西林的耐药率最高(78.9%和76.8%);其次是四环素(61.6%);对头孢唑林、头孢噻肟、头孢吡肟、庆大霉素、复方新诺明、环丙沙星和左氧氟沙星的耐药率均超过了50%;所有分离株均对亚胺培南和美罗培南敏感。其中15个分离株对受试的抗菌药物全部敏感(15.8%),63株呈多重耐药表型(66.3%)。ESBLs型菌株占53.7%。本研究探明了长春地区宠物源大肠埃希菌的耐药流行情况,对宠物临床上用药具有指导价值。
|
| 关 键 词： | 大肠埃希菌 耐药性 超广谱β内酰胺酶 宠物 |
Isolation,Identification and Antimicrobial Resistance of Escherichia coli in Companion Animals |
| |
| Abstract: | The occurrence of antimicrobial-resistant Escherichia coli and related resistance characteristics were investigated in companion animals in Changchun.E .coli were isolated and identified from 135 anal swab samples from two pet hospitals.Triplex PCR was used to detect E .coli phylogenetic groups.The re-sistance to 1 9 antimicrobial agents and extended-spectrum β-lactamases(ESBLs)phenotype were detected by BD PhoenixTM-100 System.Among 95 isolates,78.9% showed resistance to ampicillin,76.8% showed resistance to piperacillin,followed by 61.6% showed resistance to tetracycline.More than 50% isolates showed resistance to cefazolin,cefotaxime,cefepime,gentamicin,trimethoprim-sulfamethoxazole,cipro-floxacin and levofloxacin.However,all these isolates were sensitive to imipenem and meropenem.Further-more,15 isolates were sensitive to all the antimicrobials tested (15.8%).63 isolates presented multidrug resistance phenotype (66.3%).53.7% of these isolates presented ESBL phenotype.This study provided the epidemiological characteristics of antimicrobial-resistant E .coli from pets in Changchun,which may be useful for companion animal treatment. |
| |
| Keywords: | Escherichia coli antimicrobial resistance extended-spectrumβ-lactamases companion animal |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
