Molecular epidemiology and genetic linkage of macrolide and aminoglycoside resistance in Staphylococcus intermedius of canine origin |
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| Authors: | Boerlin P Burnens A P Frey J Kuhnert P Nicolet J |
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| Institution: | 1. Carol Yu Centre for Infection and Department of Microbiology, Queen Mary Hospital, University of Hong Kong, Hong Kong Special Administrative Region, People''s Republic of China;2. Department of Biomedical Sciences, College of Science and Engineering, City University of Hong Kong, Hong Kong Special Administrative Region, People''s Republic of China;1. Ruhr University Bochum, Applied Microbiology, Universitätsstraße 150, 44801 Bochum, Germany;2. ACIES BIO, Tehnoloski park 21, 1000 Ljubljana, Slovenia;3. Ruhr University Bochum, Lehrstuhl für Allgemeine und Molekulare Botanik, Universitätsstraße 150, 44801 Bochum, Germany;1. School of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, China;2. College of Environment and Energy, South China University of Technology, Guangzhou 510006, China |
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| Abstract: | A collection of 77 Staphylococcus intermedius isolates from dogs and cats in Switzerland was examined for resistance to erythromycin. Resistance profiles for 14 additional antibiotics were compared between erythromycin-resistant and susceptible isolates. A resistance prevalence of 27% for erythromycin was observed in the population under study. Complete correlation between resistance to erythromycin, and to spiramycin, streptomycin, and neomycin was observed. The erythromycin-resistant isolates all had a reduced susceptibility to clindamycin when compared to the erythromycin-susceptible isolates. Both constitutive and inducible resistance phenotypes were observed for clindamycin. Ribotyping showed that macrolide-aminoglycoside resistance was randomly distributed among unrelated strains. This suggests that this particular resistance profile is not related to a single bacterial clone but to the horizontal transfer of resistance gene clusters in S. intermedius populations. The erythromycin-resistant isolates were all carrying erm(B), but not erm(A), erm(C), or msr(A). The erm(B) gene was physically linked to Tn5405-like elements known as resistance determinants for streptomycin, streptothricin, neomycin and kanamycin. Analysis of the region flanking erm(B) showed the presence of two different groups of erm(B)-Tn5405-like elements in the S. intermedius population examined and of elements found in Gram-positive species other than staphylococci. This strongly suggests that erm(B) or the whole erm(B)-Tn5405-like elements in S. intermedius originate from other bacterial species, possibly from enterococci. |
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