| 甘肃春小麦农家品种与育成品种HMW GS变异及品质效应比较 |
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| 引用本文： | 叶春雷,王红梅,李玉芳,李望鸿.甘肃春小麦农家品种与育成品种HMW GS变异及品质效应比较[J].西北农业学报,2012,21(2):31-37. |
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| 作者姓名： | 叶春雷 王红梅 李玉芳 李望鸿 |
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| 作者单位： | 甘肃省农业科学院生物技术研究所,兰州,730070 |
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| 基金项目： | 甘肃省科技厅科学事业费项目，甘肃省中青年科学基金项目 |
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| 摘 要： | 采用SDS-PAGE法和近红外反射光谱法检测254份品种的HMW-GS和其中188份的沉淀值、蛋白质和湿面筋含量,研究甘肃春小麦育成品种和地方品种HMW-GS变异及品质效应。结果表明,共检测到21种亚基变异,46种亚基组成形式,育成品种亚基变异和组成形式不及地方品种丰富。Glu-1不同位点上,育成品种1、7+8和2+12分别为各自位点优势亚基(69.%、81.8%和86.0%),地方品种null、7+8和2+12为各自位点优势亚基(77.4%、88.7%和76.7%);育成品种1、14+15、5+10对蛋白质含量、沉淀值和湿面筋含量的效应值最高,地方品种2*、7+8和5+10对蛋白质含量的效应值最高,1、7+8和5+10亚基沉淀值和湿面筋含量的效应值最高。就优质亚基出现的频率而言,育成良品种Glu-A1位点品质改良效果较好。
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| 关 键 词： | 小麦 农家品种 高分子量谷蛋白亚基 品质效应 |
Allelic Variations of HMW GS and Their Effects on Quality of Gansu Spring Wheat Landraces and Improved Varieties |
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| YE Chunlei,WANG Hongmei,LI Yufang and LI Wanghong.Allelic Variations of HMW GS and Their Effects on Quality of Gansu Spring Wheat Landraces and Improved Varieties[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(2):31-37. |
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| Authors: | YE Chunlei WANG Hongmei LI Yufang and LI Wanghong |
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| Institution: | (Biotechnology Institute,Gansu Academy of Agriculture Science,Lanzhou 730070,China) |
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| Abstract: | Allelic variations of HMW-GS in 254 Gansu spring wheat landraces were studied by SDS-PAGE,and the quality including sedimentation,protein and wet gluten contents of 188 landraces were analyzed by near-infrared reflectance spectroscopy(NIRS) in this experiment.The results showed that 21 subunit types and 46 subunit compositions were detected in the Glu-1 loci,which implied that the landraces had much more diversities than that of the improved varieties.The subunits composition of 1(69.%),7+8(81.8%),2+12(86.0%) was the major type in the improved varieties,as well the subunits composition of null(77.4%),7+8(88.7%),2+12(76.7%) was the dominate combination in the landraces.The effect of 1,14+15,5+10 composition on quality of protein content,sedimentation and wet gluten content was the highest in the improved varieties,but the subunit combination 2*,7+8,5+10 on quality of protein content was the highest in the landraces,and 1,7+8,5+10 on quality of sedimentation and wet gluten content was the highest.The frequency of fine subunit at Glu-A1 locus was higher than that at Glu-B1 and Glu-C1 loci in the improved varieties. |
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| Keywords: | Wheat Landraces HMW-GS Quality |
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