| Ca2+对Pb2+胁迫下板蓝根种子萌发及幼苗生理特性的影响 |
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| 引用本文： | 孟红梅,寿晓晖,李彩霞.Ca2+对Pb2+胁迫下板蓝根种子萌发及幼苗生理特性的影响[J].西北农业学报,2012,21(5):185-189. |
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| 作者姓名： | 孟红梅 寿晓晖 李彩霞 |
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| 作者单位： | 河西学院农业与生物技术学院,甘肃张掖,734000 |
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| 摘 要： | 为探讨Ca2+缓解Pb2+胁迫的机制,采用培养皿发芽法,研究Pb2+(0、100、500mg/L)胁迫下不同质量浓度的Ca2+(0、80、160、320mg/L)对板蓝根种子萌发及幼苗POD、SOD活性及蛋白质质量分数的影响。结果表明,低质量浓度的Ca2+(80、160mg/L)可缓解Pb2+毒害,显著提高板蓝根种子的发芽率、发芽势、发芽指数、活力指数,促进蛋白质质量分数的增加,提高SOD、POD活性,且Ca2+80mg/L缓解效果最好,缓解能力随Pb2+质量浓度的升高有所下降;高质量浓度的Ca2+(320mg/L)与Pb2+作用,反而抑制板蓝根种子的萌发,幼苗的POD、SOD活性及蛋白质质量分数下降。低质量浓度Ca2+可以显著提高板蓝根的抗性,对Pb2+毒害起缓解作用,高质量浓度的Ca2+与Pb2+对板蓝根起协同毒害作用。
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| 关 键 词： | 板蓝根 Pb2+ Ca2+ 种子萌发 生理特性 |
The Effect of Ca2+ on Radix Isatidis Seed Germination and Seedling Physiological Characteristics under Pb2+ Stress |
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| MENG Hongmei,SHOU Xiaohui and LI Caixia.The Effect of Ca2+ on Radix Isatidis Seed Germination and Seedling Physiological Characteristics under Pb2+ Stress[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(5):185-189. |
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| Authors: | MENG Hongmei SHOU Xiaohui and LI Caixia |
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| Institution: | (College of Agriculture and Biotechnology,Hexi University,Zhangye Gansu 734000,China) |
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| Abstract: | The effects of different Ca2+ levels(0,80,160,320 mg/L) on seed germination,SOD,POD activity and protein mass fraction of Radix Isatidis seedlings under different Pb2+ stress levels(0,100,500 mg/L) were studied by petri dish germination method.The results showed that low mass concentration of Ca2+(80,160 mg/L) could ease Pb2+ poison,and significantly increase the seed germination rate,germination energy,germination index,vigour index,promote seedling SOD,POD activity and protein content,and the best result was obtained with 80 mg/L of Ca2+.The influence of Ca2+ on the Pb2+ toxicity was weakened at high Pb2+ mass concentration.Seed germination,SOD,POD activity and protein mass fraction of Radix Isatidis seedlings was inhibited under high concentration of Ca2+(320 mg/L).These result suggested that the low mass concentration of Ca2+could be advantageous against Pb2+toxicity,and could confer tolerance to Pb2+ stress in plant.The high mass concentration of Ca2+ and Pb2+ could produce synergistic toxicity on Radix Isatidis. |
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| Keywords: | Radix Isatidis Pb2+ Ca2+ Seed germination Physiological characteristics |
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