| 兔肝金属硫蛋白提取液的稳定性 |
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| 引用本文： | 党蕊叶,蒋志武,王开锋,权清转,权启俊.兔肝金属硫蛋白提取液的稳定性[J].西北农业学报,2012,21(3):42-45. |
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| 作者姓名： | 党蕊叶 蒋志武 王开锋 权清转 权启俊 |
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| 作者单位： | 1. 陕西省动物研究所,西安,710032
2. 陕西同腾生物科技有限公司,陕西澄城,715200 |
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| 基金项目： | 陕西省科学院重大项目(2010k-01-z4);陕西省农业攻关计划项目(2011K02-08) |
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| 摘 要： | 研究Tris-HCl浓度、pH、温度、光照和乙醇对金属硫蛋白提取液稳定性的影响,为金属硫蛋白的研究提供资料.结果表明,金属硫蛋白在低浓度Tris-HCl中较稳定,0.01和0.02 mol/L Tris-HCl对其稳定性影响最小；pH对金属硫蛋白稳定性影响较大,pH 6～7时较稳定;光照对金属硫蛋白稳定性影响比较明显,其对硫蛋白具有破坏作用；乙醇对金属硫蛋白有明显的保护作用；金属硫蛋白具有很强的耐热性.
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| 关 键 词： | 金属硫蛋白 稳定性 高温 浓度 pH 光照 乙醇 |
Stability of Metallothionein Extracted from Livers of Rabbit |
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| DANG Ruiye,JIANG Zhiwu,WANG Kaifeng,QUAN Qingzhuan and QUAN Qijun.Stability of Metallothionein Extracted from Livers of Rabbit[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(3):42-45. |
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| Authors: | DANG Ruiye JIANG Zhiwu WANG Kaifeng QUAN Qingzhuan and QUAN Qijun |
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| Institution: | 1.Shaanxi Institute of Zoology,Xi’an 710032,China; 2.Shaanxi Tongteng Biotechnology Ltd.,Chengcheng Shaanxi 715200,China) |
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| Abstract: | The stability of metallothionein(MT) extracted from livers of rabbit was conducted in this paper affected by consistence of Tris-HCl,pH,high temperature,illumination and ethanol.The experiment results show: the MT is not stable in higher Tris-HCl buffer solution,and 0.01 mol/L and 0.02 mol/L Tris-HCl,which are appropriate MT-extracted consistence,have less effect on the stability of MT;the range of feasible MT-extracted pH is 6-7;illumination accelerates MT transformation;ethanol has good protect role on MT;and MT has stronger heat resistance,high temperature in short time has little effect on stability of MT. |
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| Keywords: | Metallothionein Stability High temperature Tris-HCl pH Illumination Ethanol |
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