| 几种理化因子对青钱柳悬浮细胞生长的影响 |
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| 引用本文： | 任民红,上官新晨,陈继光,蒋 艳,吴少福,尹忠平.几种理化因子对青钱柳悬浮细胞生长的影响[J].西北农业学报,2012,21(5):179-184. |
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| 作者姓名： | 任民红 上官新晨 陈继光 蒋 艳 吴少福 尹忠平 |
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| 作者单位： | 江西农业大学食品科学与工程学院,江西省高等学校天然产物研究与开发重点实验室,南昌330045 |
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| 基金项目： | 江西省主要学科学术和技术带头人项目 |
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| 摘 要： | 为了利用细胞悬浮培养技术生产青钱柳细胞及其代谢物质以解决青钱柳资源稀缺问题,采用单因素和正交试验优化青钱柳细胞悬浮培养条件。结果表明,在供试的WPM、MS、1/2MS、B5、N6、DKW、White7种培养基中,WPM、MS、B5均有利于悬浮细胞的生长;有利于悬浮细胞生长的碳源是果糖和蔗糖;2,4-D是影响青钱柳悬浮细胞生长的关键因子,对细胞生长影响极显著,最佳激素组合为0.4mg/L 2,4-D+0.4mg/LNAA+0.4mg/L KT+0.4mg/L 6-BA;通过L9(34)正交试验得出几种物理因子对悬浮细胞生长的影响效应排序为接种量>装液量>转速>pH,最佳条件组合为接种量80g/L、装液量40%、摇床转速115r/min、pH 5.0。
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| 关 键 词： | 青钱柳 悬浮培养 正交设计 激素配比 |
Effects of Several Factors on the Growth of Cyclocarya paliurus Suspension Cells |
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| REN Minhong,SHANGGUAN Xinchen,CHEN Jiguang,JIANG Yan,WU Shaofu and YIN Zhongping.Effects of Several Factors on the Growth of Cyclocarya paliurus Suspension Cells[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(5):179-184. |
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| Authors: | REN Minhong SHANGGUAN Xinchen CHEN Jiguang JIANG Yan WU Shaofu and YIN Zhongping |
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| Institution: | REN Minhong
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SHANGGUAN Xinchen
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CHEN Jiguang
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JIANG Yan
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WU Shaofu
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Y1N Zhongping |
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| Abstract: | In order to solve the scarcity of Cyclocarya paliurus resources,the cell suspension culture technology was used to produce C.paliurus cells and its metabolites.The optimal growing conditions of suspension cells were investigated by single factor and orthogonal experiment.The results showed as follows: among the seven basic medium of WPM,MS,1/2MS,B5,N6,DKW and White tested,WPM,MS and B5 were favorable for the growth of suspension cells.Sucrose and fructose were favorable for the growth of suspension cells.2,4-D was the key factor for the growth of suspension cells.Suspension cells grew well in MS medium supplemented with 0.4 mg/L 2,4-D,0.4 mg/L NAA,0.4 mg/L KT and 0.4 mg/L 6-BA.Experimental results indicated that the impact order of four factors on the growth was listed as follows: inoculum densities >liquid volume > rotation speed >initial pH,the optimal culture condition combination was 8% of Inoculum densities,40% of liquid medium volume,115 r/min of rotation speed and initial pH 5.0. |
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| Keywords: | Cyclocarya paliurus Suspension culture Orthogonal design Hormone combination |
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