| 酒酒球菌膜脂肪酸成分分析的样品处理方法比较 |
| |
| 引用本文： | 吕 茜,张国强,樊明涛.酒酒球菌膜脂肪酸成分分析的样品处理方法比较[J].西北农业学报,2012,21(1):182-197. |
| |
| 作者姓名： | 吕 茜 张国强 樊明涛 |
| |
| 作者单位： | 西北农林科技大学食品学院,陕西杨凌,712100 |
| |
| 基金项目： | 国家葡萄产业技术体系专项 |
| |
| 摘 要： | 为筛选较适合的酒酒球菌膜脂肪酸提取和皂化方法,采用5种不同脂肪酸分析样品前处理方法,结合气相色谱质谱联用仪(GC/MS),分析酒酒球菌SD-2a膜脂肪酸成分,比较不同处理方法的效果。结果表明,先提取脂肪酸再采用NaOCH3甲酯化脂肪酸的方法能够较为准确和完整地检出酒酒球菌SD-2a的12种膜脂肪酸,相对标准误差(RSD)≤5%,特别对于环丙烷脂肪酸的检测效果较好,RSD=2%;独立的脂肪酸提取步骤对细菌膜脂肪酸分析是必要的,包含脂肪酸提取的样品处理方法比不包含脂肪酸提取的方法多检出3种脂肪酸,RSD均≤5%。
|
| 关 键 词： | 酒酒球菌 膜脂肪酸 甲酯化 脂肪提取 |
Comparing Sample Preparation Methods for the Analysis of Membrane Fatty Acid Composition of Oenococcus oeni |
| |
| L Qian
,
ZHANG Guoqiang
,
FAN Mingtao.Comparing Sample Preparation Methods for the Analysis of Membrane Fatty Acid Composition of Oenococcus oeni[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(1):182-197. |
| |
| Authors: | L Qian ZHANG Guoqiang FAN Mingtao |
| |
| Institution: | L(U) Qian
,
ZHANG Guoqiang
,
FAN Mingtao |
| |
| Abstract: | Five different methods combined with GC/MS analysis of membrane fatty acid composition of Oenococcus oeni SD-2a were compared.This study validated that the method including lipids extraction and NaOCH3 methylation was more accurate and efficient,by which 12 fatty acids were detected,RSD is less than 5%,and it was suitable particularly for analysis of cyclopropane fatty acids such as C19cyc11 fatty acid,with a 2% RSD;the study also indicated that the lipids extraction procedure prior to the methylation of samples cannot be omitted.Three more fatty acids,in which RSD were all less than 5%,were detected in the method involving lipids extraction procedure than the method without lipids extraction. |
| |
| Keywords: | Oenococcus oeni Membrane fatty acid Methylation Lipids extraction |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
|
