| 西藏拟溞遗传多样性的初步研究 |
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| 引用本文： | 赵文,毕进红,韩婷婷,魏杰,柴晓杰,芦佳轩,邢跃楠.西藏拟溞遗传多样性的初步研究[J].大连水产学院学报,2011,26(2):108-113. |
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| 作者姓名： | 赵文 毕进红 韩婷婷 魏杰 柴晓杰 芦佳轩 邢跃楠 |
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| 作者单位： | 大连海洋大学辽宁省水生生物学重点实验室;大连海洋大学生命科学与技术学院; |
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| 基金项目： | 国家自然科学基金资助项目(30671625,40776065,31072210) |
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| 摘 要： | 利用随机扩增多态性DNA（RAPD）技术对西藏拟溞Daphniopsis tibetana4个群体（新疆赛里木湖、西藏色林错、纳木卡错和班戈湖）的遗传多样性进行了初步研究,用21个随机引物对4个群体80个样本进行了随机扩增,从中筛选出5个扩增效果比较稳定的引物,共得到多态位点为25个。利用POPGENE32分析表明,西藏拟溞各群体遗传变异由高向低依次为色林错（SLC）〉赛里木湖（SLM）〉纳木卡错（NMKC）〉班戈湖（BGH）。根据遗传距离结果,采用UPGMA和NJ方法分别进行聚类分析,结果表明,赛里木湖（SLM）和色林错湖（SLC）两群体亲缘关系较近,纳木卡错（NMKC）与班戈湖（BGH）两群体之间的亲缘关系比较近。
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| 关 键 词： | 西藏拟溞 遗传多样性 RAPD |
Genetic diversity of four populations of Cladocera Daphniopsis tibetana |
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| ZHAO Wen,BI Jin-hong,HAN Ting-ting,WEI Jie,CHAI Xiao-jie,LU Jia-xuan,XING Yue-nan.Genetic diversity of four populations of Cladocera Daphniopsis tibetana[J].Journal of Dalian Fisheries University,2011,26(2):108-113. |
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| Authors: | ZHAO Wen BI Jin-hong HAN Ting-ting WEI Jie CHAI Xiao-jie LU Jia-xuan XING Yue-nan |
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| Institution: | ZHAO Wen1,2,BI Jin-hong1,HAN Ting-ting1,WEI Jie1,CHAI Xiao-jie1,LU Jia-xuan1,XING Yue-nan1(1.Key Laboratory of Hydrobiology in Liaoning Province,Dalian Ocean University,Dalian 116023,China,2.School of Life Science and Technology,China) |
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| Abstract: | The genetic diversity of four populations(Selincuo in Tibet,SLC;Namukacuo in Tibet,NMKC;Bangehu in Tibet,BGH;Sailimuhu in Xinjiang,SLM) of Daphniopsis tibetana Sars were studied by RAPD technique.Twenty-one random primers were used to test eighty samples of four populations,and five primers were chosen for generation of a total of 51 high steady bands.The order of genetic variations analyzed by POPGENE32 in the four populations were arranged as SLC>SLM>NMKC>BGH.The phylogenetic tree among 4 populations cons... |
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| Keywords: | Daphniopsis tibetana genetic diversity RAPD |
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