| 一个水稻茎叶优势表达锌指基因启动子的克隆及功能分析 |
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| 引用本文： | 郑娅珊,袁国强,刘华清,王锋.一个水稻茎叶优势表达锌指基因启动子的克隆及功能分析[J].福建农业学报,2009,24(1). |
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| 作者姓名： | 郑娅珊 袁国强 刘华清 王锋 |
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| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350007;福建省农业科学院福建农业遗传工程重点实验室,福建,福州,350003
2. 福建省农业科学院福建农业遗传工程重点实验室,福建,福州,350003 |
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| 摘 要： | 通过RT-PCR分析,鉴定了一个在水稻茎、叶中特异表达的锌指基因OsZF153.利用PCR扩增了OsZF153基因上游约2 kb启动子区,构建了启动子与GUS的融合表达载体pCZF153P-GUS.pCZF153P-GUS转基因T0植株GUS活性分析显示:转基因植株的根、茎、叶、颖壳中检测到GUS活性,其中茎和叶GUS表达活性较强,而根和颖壳表达较弱,在雄蕊和雌蕊及种子不同发育阶段中没有检测到GUS活性,表明OsZF153启动子是一个水稻非生殖器官表达,且在茎、叶中优势表达的启动子.
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| 关 键 词： | 水稻 锌指基因 茎叶 优势表达 |
Cloning and functions of a zinc-finger gene promoter preferentially expressed in rice stems and leaves |
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| ZHENG Ya-shan,YUAN Guo-qiang,LIU Hua-qing,WANG Feng.Cloning and functions of a zinc-finger gene promoter preferentially expressed in rice stems and leaves[J].Fujian Journal of Agricultural Sciences,2009,24(1). |
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| Authors: | ZHENG Ya-shan YUAN Guo-qiang LIU Hua-qing WANG Feng |
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| Institution: | 1.College of Life Sciences;Fujian Normal University;Fuzhou;Fujian 350007;China;2.Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture;Fujian Academy of Agricultural Sciences;Fujian 350003;China |
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| Abstract: | A zinc-finger gene(tentatively named OsZF153),which was preferentially expressed in the rice stems and leaves,was identified by RT-PCR analysis.A fusion vector was constructed by combining approximately 2 kb upstream from the start codon of the gene OsZF153 with the reporter gene GUS.The GUS activity was detected in the T0 generation of transgenic rice plants.A strong GUS activity could be detected in the stems and leaves,while a weak one in the roots and hull,of the genetically modified rice.But no GUS act... |
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| Keywords: | OsZF153 |
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