| 长豇豆SSR有效引物的筛选与反应程序的确定 |
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| 引用本文： | 彭海,张静,徐凡,陈禅友.长豇豆SSR有效引物的筛选与反应程序的确定[J].湖北农业科学,2007,46(4):504-507. |
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| 作者姓名： | 彭海 张静 徐凡 陈禅友 |
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| 作者单位： | 江汉大学生命科学学院,武汉,430056 |
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| 基金项目： | 湖北省武汉市科技攻关项目 |
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| 摘 要： | 对长豇豆有效SSR引物进行了筛选,共有13对引物能对长豇豆基因组进行有效扩增,并分别确定了其适合的PCR反应程序.还对长豇豆SSR引物数量及豇豆中开发的SSR引物在长豇豆中的适用性进行了讨论.为利用SSR分子标记分析长豇豆遗传关系奠定了基础.
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| 关 键 词： | 长豇豆 微卫星标记 引物 |
| 文章编号： | 0439-8114（2007）04-0504-03 |
| 修稿时间： | 2007-04-13 |
Screening for Effective SSR Primers of Asparagus Bean and the Determination of Its Reaction Procedures |
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| PENG Hai,ZHANG Jing,XU Fan,CHEN Chan-you.Screening for Effective SSR Primers of Asparagus Bean and the Determination of Its Reaction Procedures[J].Hubei Agricultural Sciences,2007,46(4):504-507. |
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| Authors: | PENG Hai ZHANG Jing XU Fan CHEN Chan-you |
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| Institution: | College of Life Sciences, Jianghan University, Wuhan 430056, China |
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| Abstract: | The effective primers in asparagus bean SSR amplification were screened in this paper. A total of 13 primers were proved to be effective and its proper PCR procedures were also obtained. The issues, such as the SSR primer number and the transferability of SSR primers developed in cowpea to asparagus bean, were discussed. A foundation for the genetic analysis of asparagus bean with SSR technique was set up. |
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| Keywords: | asparagus bean SSR marker primer |
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