| 鸡毒支原体PCR检测方法的建立 |
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| 引用本文： | 秦璐璐.鸡毒支原体PCR检测方法的建立[J].动物医学进展,2009,30(2). |
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| 作者姓名： | 秦璐璐 |
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| 作者单位： | 安徽农业大学动物科技学院,安徽合肥,230036 |
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| 摘 要： | 根据已发表的鸡毒支原体种特异性序列fMG-2设计1对引物,建立检测鸡毒支原体的PCR方法.该法对鸡毒支原体能特异性扩增726 bp的目的片段,而对其他禽病原DNA模板的扩增结果为阴性.建立的PCR方法对鸡毒支原体的最少检出量为3 Pg.用建立的PCR方法对临床采集的样品进行检测,同时对相应的样品进行细菌分离,结果临床样品PCR的阳性检出率为20.5%,细菌分离培养的阳性率为0.9%,表明PCR的敏感性高于细菌分离鉴定.
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| 关 键 词： | 鸡毒支原体 聚合酶链反应 检测 分离鉴定 |
Establishment of PCR for Detection of Mycoplasma gallisepticum |
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| QIN Lu-lu.Establishment of PCR for Detection of Mycoplasma gallisepticum[J].Progress In Veterinary Medicine,2009,30(2). |
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| Authors: | QIN Lu-lu |
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| Institution: | College of Animal Sicence and Technologe;Anhui Agriculture University;Hefei;Anhui;230036;China |
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| Abstract: | A pair of primers was designed based on fMG-2 sequences of avian Mycoplasma.The primers selectively amplified a 726 bp product from Mycoplasma gallisepticum(MG),but did not amplify the DNA or RNA of other bacteria or viruses.The detection threshold of PCR for MG was 3 pg.The samples detected by PCR,and isolation and identification.Using PCR,20.5% of field samples was positively detected,it was higher than that of isolation and identification.The results indicated that this technique is a useful screening as... |
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| Keywords: | Mycoplasma gallisepticum PCR detection isolation and identification |
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