| 利用EST同工酶谱鉴定黑稻品种纯度的研究 |
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| 引用本文： | 丁锐,李利华.利用EST同工酶谱鉴定黑稻品种纯度的研究[J].种子,2006,25(4):21-22,26. |
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| 作者姓名： | 丁锐 李利华 |
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| 作者单位： | 陕西省资源生物重点实验室,汉中,723000 |
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| 摘 要： | 采用改进的聚丙烯酰胺凝胶电泳对5种黑稻主要栽培品种进行了EST同工酶谱分析.结果表明,此方法分辨率高.测试各品种的EST同工酶存在丰富的多态性,其电泳图谱具有鲜明的特征,并构成了各自的＂指纹＂.其中黑优粘和云黑酶谱条带数多达15条,a1和a3为5个品种的共有谱带.通过EST同工酶谱相似度指数分析,黑优粘与黑丰糯、乌贡1号相似度指数大于0.75,说明这3个品种间亲缘关系较近.
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| 关 键 词： | 黑稻 EST同工酶谱 纯度检测 亲缘关系 |
| 收稿时间： | 2005-12-30 |
| 修稿时间： | 2005-12-30 |
Study on Purity of Black Rices Variety with EST Isozyme |
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| Ding Rui,Li Lihua.Study on Purity of Black Rices Variety with EST Isozyme[J].Seed,2006,25(4):21-22,26. |
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| Authors: | Ding Rui Li Lihua |
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| Institution: | Bio-resources Key laboratory of Shanxi Province, Hanzhong,723000 |
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| Abstract: | Analysis of the Esterase isozyme of black rices variety was carried out by PAGE,The results showed that the Esterase isozyme had a plenty of polymorphism on account of high resolution.Each variety of black rices has it's own unique band pattern.Compared with their finger prints,the results showed their bands reach 15 at most.a_1 and a_3 bands are common for five varieties.Analysis of similarity degree index of EST Isozyme indicated that three varieties had a near relationship becanse of a high index which is above 0.75. |
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| Keywords: | Black rice Esterase isozyme Relationship Purity identification |
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