| 同一病株西瓜枯萎病菌RAMS分析 |
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| 引用本文： | 段会军,姚占军,刘 卫,关占良.同一病株西瓜枯萎病菌RAMS分析[J].中国农学通报,2006,22(2):72-72. |
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| 作者姓名： | 段会军 姚占军 刘 卫 关占良 |
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| 作者单位： | 沈阳农业大学园艺学院 辽宁沈阳110161 |
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| 摘 要： | 利用RAMS (Random amplified microsatellites)分子标记技术对河北省不同地区的3个西瓜枯萎病病株分离物Fon1、Fon2、Fon3各10个单孢菌株进行了基因组多态性分析。10个RAMS引物进行扩增， Fon1共扩增出39条带，其中多态性条带9条；Fon2共扩增出40条带，多态性条带5条；Fon3共扩增出40条带，多态性条带4条。结果分析表明，同一病株分离物的不同单孢菌株之间在分子水平上存在遗传差异性。
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| 关 键 词： | 西瓜 枯萎病菌 RAMS |
| 收稿时间： | 2005-09-29 |
| 修稿时间： | 2005-09-292005-10-09 |
Analysis on Fusarium oxyporum f.sp. niveum of the Same Disease Plant by RAMS |
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| Duan Huijun,Yao Zhanjun,Liu Wei,Guan Zhanliang.Analysis on Fusarium oxyporum f.sp. niveum of the Same Disease Plant by RAMS[J].Chinese Agricultural Science Bulletin,2006,22(2):72-72. |
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| Authors: | Duan Huijun Yao Zhanjun Liu Wei Guan Zhanliang |
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| Institution: | College of Horticulture, Shenyang Agricultural University, Shenyang 11016 |
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| Abstract: | The bulb scales and bulblets culture in vitro of Lilium lancifolium Thunb were studied. The sterilization time, inoculation methods and the effect of plant hormones on inducement and proliferation were compared. The results showed 70% alcohol with 20s and 0.1% Hgcl2 with 12min was the best for the bulb scales sterilization and 70% alcohol with 20s, 0.1% HgCl2 with 10min for bulblets. The regeneration ability of external scales was better than the middle ones, which was better than the internal ones. MS 6-BA1.5mg/L NAA0.2mg/L could induce more and stronger shoots. The best proliferation medium was MS 6-BA1.0mg/L NAA0.2mg/L. The multiplication coefficient was 8.0 with strong plantlets. |
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| Keywords: | Lilium lancifolium Thunb Bulb Scales Bulblets Tissue Culture |
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