| 日粮核苷酸对早期断奶小鼠肝脏基因表达的影响 |
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| 引用本文： | 王兰芳,乐国伟,虞泽鹏,施用晖.日粮核苷酸对早期断奶小鼠肝脏基因表达的影响[J].上海交通大学学报(农业科学版),2006,24(4):341-344,348. |
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| 作者姓名： | 王兰芳 乐国伟 虞泽鹏 施用晖 |
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| 作者单位： | 1. 同济大学,基础医学院,上海,200092
2. 江南大学,食品学院,无锡,214036 |
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| 基金项目： | 教育部高校骨干教师资助计划 |
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| 摘 要： | 研究了日粮核苷酸对早期断奶小鼠肝脏基因表达的影响:选取16～17日龄健康BALB/C雄性小鼠60只,分成对照组和实验组,分别饲喂添加0、0.25%核苷酸的无核苷酸基础日粮,在实验的第2、4、10天采用差异显示技术分离小鼠肝脏中差异表达的基因。结果表明:采用6对引物分离到4条差异表达的基因片断。采用差异显示技术初步分离到4个受核苷酸影响差异表达的基因,这些片段需要做进一步的验证。
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| 关 键 词： | 核苷酸 差异显示 小鼠 |
| 文章编号： | 1671-9964（2006）04-0341-04 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-03-10 |
Effects of Dietary Nucleotides on Hepatic Gene Expression of Early-weaned Mice |
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| WA NG Lan-fang,LE Guo-wei,YU Ze-peng,SHI Yong-hui.Effects of Dietary Nucleotides on Hepatic Gene Expression of Early-weaned Mice[J].Journal of Shanghai Jiaotong University (Agricultural Science),2006,24(4):341-344,348. |
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| Authors: | WA NG Lan-fang LE Guo-wei YU Ze-peng SHI Yong-hui |
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| Institution: | 1. School of medicine, Tongji Univ., Shanghai 200092; 2. School of Food Science and Technology, Southern Yangtze Univ., Wuxi 214036, China |
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| Abstract: | Objective: To isolate differential expressed genes induced by exogenous nucleotides in Early-weaned mice. Methods: 60 Balb/C male mice, 16~17 days, were divided into two treatments (fed 0?,0.25% nucleotide-supplemented diet respectively). Mice were killed at 2 d, 4 d, 10 d and liver were collected for mRNA differential display to isolate differential expressed genes. Results: 4 differential fragments were obtained by second PCR amplifying. Two of which were expressed in control group and the others were expressed in test group. Conclusion: 4 differential fragments influenced by exogenous nucleotides were obtained. These fragments need to be identified by northern blot, cloning, sequencing and homology analysis. |
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| Keywords: | nucleotides DDRT-PCR mice |
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