| 3种市售养殖对虾白斑综合征病毒(WSSV)的核酸探针检测 |
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| 引用本文： | 闫冬春,杨雪,于谨磊,刘路清,杜中梅,时良,王芳.3种市售养殖对虾白斑综合征病毒(WSSV)的核酸探针检测[J].水生态学杂志,2008,29(2):58-61. |
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| 作者姓名： | 闫冬春 杨雪 于谨磊 刘路清 杜中梅 时良 王芳 |
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| 作者单位： | 鲁东大学生命科学学院 山东烟台;鲁东大学生命科学学院 山东烟台;鲁东大学生命科学学院 山东烟台;鲁东大学生命科学学院 山东烟台;鲁东大学生命科学学院 山东烟台;鲁东大学生命科学学院 山东烟台;鲁东大学生命科学学院 山东烟台 |
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| 摘 要： | 对虾白斑综合征病毒是对虾养殖业危害最为严重的病毒, 每年给对虾养殖造成很大经济损失, 已成为对虾养殖业可持续发展的严重障碍。在过去的十几年中, 人们采取各种方法防止白斑综合征病毒的传播。中国明对虾(Fenneropenaeus chinensis )、凡纳滨对虾( Litopenae vannamei)、日本囊对虾(Marsup enaeus jap onicus)是我国主要的对虾养殖品种, 也是白斑综合征病毒的敏感宿主。按照煮沸- 乙醇沉淀法快速、简便提取市售30 只中国明对虾、28只凡纳滨对虾、29只日本囊对虾DNA, 然后用地高辛标记的核酸探针进行斑点杂交检测白斑综合征病毒,从而了解市场中养殖对虾携带白斑综合征病毒的情况。检测结果显示, 所有样品均未感染白斑综合征病毒, 说明目前白斑综合征病毒在一定程度上得到了有效控制。
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| 关 键 词： | 中国明对虾 凡纳滨对虾 日本囊对虾 白斑综合征病毒 核酸探针 |
White Spot Syndrome Virus (WSSV) Detection with Nucleic Probe in Three Cultured Shrimps |
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| YAN Dong-chun,YANG Xue,YU Jin-lei,LIU Lu-qing,DU Zhong-mei,SHI Liang and WANG Fang.White Spot Syndrome Virus (WSSV) Detection with Nucleic Probe in Three Cultured Shrimps[J].Journal of Hydroecology,2008,29(2):58-61. |
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| Authors: | YAN Dong-chun YANG Xue YU Jin-lei LIU Lu-qing DU Zhong-mei SHI Liang and WANG Fang |
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| Institution: | Department of Life and Science, Ludong University , Yantai , Shandong 264025 , China,Department of Life and Science, Ludong University , Yantai , Shandong 264025 , China,Department of Life and Science, Ludong University , Yantai , Shandong 264025 , China,Department of Life and Science, Ludong University , Yantai , Shandong 264025 , China,Department of Life and Science, Ludong University , Yantai , Shandong 264025 , China,Department of Life and Science, Ludong University , Yantai , Shandong 264025 , China and Department of Life and Science, Ludong University , Yantai , Shandong 264025 , China |
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| Abstract: | White spot syndrome virus (WSSV) was considered the most serious virus for shrimp aquaculture. This virus has brought great yearly economic losses in world shrimp farming and has become a major deterrent in the growth and sustainability of shrimp aquaculture . In the past more than 10 years , many techniques have been used to prevent this virus' transmission. Fenneropenaeus chinensis , Litopenaeus vannamei , Marsupenaeus japonicus are three kinds of popular cultured shrimp in China. They are also WSSV acute hosts. In this paper , DNA of 30 Fenneropenaeus chinensis , 28 Litopenaeus vannamei ,29 Marsupenaeus japonicus bought from seafood market were extracted rapidly by applying the method of boiling - ethanol precipitation , and then detected by digoxigenin - labeled mucleic probe dot blot hybridization for white spot syndrome virus carrying status. The results showed that all of the samples were WSSV negative , which indicated that WSSV was controlled effectively to some degree in China. |
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| Keywords: | Fennerpenaeus chinensis Litopenaeus vannamei Marsupenaeus japonicus white spot syndrome virus(WSSV) nucleic probe |
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