Abstract: | Investigations were conducted to evaluate the effects of long-term (up to 100 years) wastewater irrigation on the selected microbiological and biochemical characteristics of a Cambisol and a Haplic Luvisol. The developments of soil oligotrophic and copiotrophic bacteria (including actinomycetes), the total microbial biomass (ATP content), and acitivity of enzymes catalyzing major substrate transformations in soil (β-glucosidase, β-acetyl-glucosaminidase, proteinase, phosphatase) indicated significantly differences between wastewater treated and non-irrigated soil sites. The increases of the individual indices under the effect of wastewater irrigation show that remarkable differences in the microbiological and biochemical parameters of soil quality remained detectable also 20 years after the soil treatment was terminated. |