| 贵州白香猪的遗传检测 |
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| 引用本文： | 于冰,刘若余,刘培琼.贵州白香猪的遗传检测[J].家畜生态,2009(3). |
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| 作者姓名： | 于冰 刘若余 刘培琼 |
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| 作者单位： | 贵州大学动物科学学院省动物遗传育种与繁殖重点实验室; |
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| 基金项目： | 贵州省农业厅地方畜禽保护项目资助 |
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| 摘 要： | 选取27个微卫星位点,应用PCR技术对贵州白香猪的2个品系进行遗传质量监测。结果在所选的27个微卫星位点中,共有24个位点获得稳定的扩增结果,其中SW911位点与S0026两个位点在群体内表现为单态纯合。2个品系有效等位基因、平均多态信息含量、平均杂合度、基因纯合率和平均近交系数分别为1.8310、1.7951、0.3436、0.3249、0.4329、0.4188、52.63%、58.55%、0.5377、0.5605。结果表明微卫星标记可用于检测贵州白香猪的遗传质量,也可用于分析封闭群的遗传多态性。是一种有潜力的试验动物遗传学检测标记。
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| 关 键 词： | 贵州白香猪 微卫星 遗传检测 |
Genetic Detection of Guizhou University White Xiang Pig |
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| YU Bing,LIU Ruo-yu,LIU Pei-qiong.Genetic Detection of Guizhou University White Xiang Pig[J].Ecology of Domestic Animal,2009(3). |
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| Authors: | YU Bing LIU Ruo-yu LIU Pei-qiong |
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| Institution: | Guizhou Key Lab of Animal Genetics;Breeding and Reproduction;College of Animal Sciences;Guizhou University;Guiyang 550025;China |
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| Abstract: | 27 microsatellites loci were selected to monitor the genetic quality in two strains Guizhou University white Xiang Pig by PCR.Results 24 microsatellites DNA could be amplified efficiently.It displayed homozygosis in SW911 and S0026 1oci.Results showed that: the number of effective allele,PICs,the gene heterozygosity,the gene homogeneity index and inbreeding coefficients of two breeds strains were 1.8310,1.7951;0.3436,0.3249;0.4329,0.4188;52.63%,58.55% and 0.5377,0.5605,respectively.The microsatellite marker... |
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| Keywords: | Guizhou University White Xiang Pig microsatellite DNA genetic detection |
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