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斜带髭鲷血液生理和生化指标的研究
引用本文:齐红莉,梁拥军,杨广,刘金兰.斜带髭鲷血液生理和生化指标的研究[J].安徽农业科学,2009,37(29):14210-14212.
作者姓名:齐红莉  梁拥军  杨广  刘金兰
作者单位:齐红莉,杨广,刘金兰(天津农学院水产科学系,天津市水产生态及养殖重点实验室,天津,300384);梁拥军(北京市水产科学研究所,北京,100068) 
基金项目:天津市科技攻关项目,天津农学院教育教学研究与改革项目 
摘    要:[目的] 丰富斜带髭鲷血液生理生化指标的数据库。方法]以60尾斜带髭鲷成鱼为材料,运用常规血液指标和血细胞涂片方法,测定了血液生理、生化指标。[结果]斜带髭鲷成鱼血液中,红细胞和白细胞数量分别为(215.25±14.33)万和(1.87±0.43)万个/mm^3;血红蛋白含量为(62.30±7.50)g/L;红细胞压积为(23.3±1.01)%;红细胞沉降率为(0.58±0.21) mm/h。血涂片经显微镜观察显示可以明显分出血红细胞、淋巴细胞、单核细胞、血栓细胞、嗜中性粒细胞。血液中的血清总蛋白、白蛋白和肌酐浓度分别为(32.13±8.61)、(26.46±2.75)和(16.15±3.86)μmol/L;乳酸脱氢酶活性为(1 446.30±523.58)IU/L;磷酸脱氢酶活性为(30.33±13.92)IU/L;甘油三酯为(1.80±0.46)mmol/L;总胆固醇为(2.68±0.51)mmol/L;Ca^2+、Na^+、Cl^-和K^+浓度分别为(1.31±0.22)、(191.74±5.27)、(154.14±8.51)和(2.11±0.58) mmol/L。[结论]该试验为斜带髭鲷鱼的基础生物学研究提供血液学基础资料。

关 键 词:斜带髭鲷  血液  生理指标  生化指标

Studies on the Physiological and Biochemical Indices of Blood in Hapalogenys nitens
QI Hong-li et al.Studies on the Physiological and Biochemical Indices of Blood in Hapalogenys nitens[J].Journal of Anhui Agricultural Sciences,2009,37(29):14210-14212.
Authors:QI Hong-li
Institution:QI Hong-li et al (Department of Fishery Science,Tianjin Agricultural University,Key Laboratory of Aquatic Ecology , Aquaculture of Tianjin,Tianjin 300384)
Abstract:Objective] The study aimed to rich the database of blood physiological and biochemical indexes of Hapalogenys nitens.Method] With 60 trails of adult H.nitens as the tested materials,their blood physiological and biochemical indexes in blood were determined by using conventional blood indexes and blood corpuscle smear method.Result] In the adult fish of H.nitens,the erythrocyte and leukocyte counts were(215.25±14.33)×104 /mm3 and(1.87±0.43)×104 /mm3,resp.;the count of hemoglobin was(62.30±7.50) g/L,the hematokrit was(23.3%±1.01)% and the sedimentation rate of erythrocyte was(0.58±0.21) mm/h.Erythrocyte,lymphocyte,monocyte,thrombocytes and neutrophilic granulocyte were recognized on the stained smears by microscope observation.The contents of TP,AP and CRE in serum were(32.13±8.61),(26.46±2.75) and(16.15±3.86) μmol/L,resp.;the activity of LDH and AKP were(1 446.30±523.58) and(30.33±13.92) IU/L,resp.;the content of TG and CHOL were(1.80±0.46) and(2.68±0.51) mmol/L;the contents of Ca2+,Na+,Cl-and K+ in serum were(1.31±0.22),(191.74±5.27),(154.14±8.51) and(2.11±0.58) mmol/L,resp.Conclusion] This study provided the foundational basis for hematology in the research on the basic biology of H.nitens.
Keywords:Hapalogenys nitens  Blood  Physiological index  Biochemical parameters  
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