| 质粒DNA拓扑学结构对基因疫苗生物学特性的影响 |
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| 引用本文： | 李作生,余兴龙,张茂林,乔红伟,程从升,徐兴然,扈荣良,涂长春.质粒DNA拓扑学结构对基因疫苗生物学特性的影响[J].中国兽医学报,2004,24(3):250-251. |
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| 作者姓名： | 李作生 余兴龙 张茂林 乔红伟 程从升 徐兴然 扈荣良 涂长春 |
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| 作者单位： | 解放军军需大学,军事兽医研究所,吉林,长春,130062 |
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| 基金项目： | 国家“8 63”计划项目 ( 2 0 0 1AA2 13 14 1) |
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| 摘 要： | 利用不同拓扑学结构的质粒 ,分别转化 E.coli感受态细胞、转染 SK6 - 6细胞和免疫小鼠 ,比较了不同拓扑学结构质粒对转化效率、转染率和对免疫应答水平的影响。结果显示 ,超螺旋占 5 0 %比例的猪瘟病毒 E2基因表达质粒p CDSW转化 E.coli感受态细胞的效率比开环的 p CDSW高 8倍 ,超螺旋比例占 80 %的 p VAXE2 IL- 2质粒比酶切线性化质粒转化率高 2 2倍 ;以荧光蛋白基因为报告基因 ,超螺旋比例占 80 %的荧光蛋白真核表达质粒转染真核细胞的效率是线性化质粒的 12倍 ;以猪瘟病毒主要保护性抗原 E2基因表达质粒 ,分别制备成超螺旋和开环形式占优势的质粒 ,免疫昆明鼠 ,抗体检测结果显示 ,超螺旋比例高的基因疫苗免疫效果明显好于开环质粒的基因疫苗
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| 关 键 词： | 质粒DNA 拓扑学结构 猪瘟病毒 基因疫苗 生物学特性 |
| 文章编号： | 1005-4545(2004)03-0250-02 |
| 修稿时间： | 2003年5月20日 |
Effect of Plasmid DNA toplogy on Biologic Property of DNA Vaccine |
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| LI Zuo-sheng,YU Xing-long,ZHANG Mao-lin,QIAO Hong-wei,CHENG Cong-sheng,XU Xing-ran,HU Rong-liang,TU Chang-chun.Effect of Plasmid DNA toplogy on Biologic Property of DNA Vaccine[J].Chinese Journal of Veterinary Science,2004,24(3):250-251. |
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| Authors: | LI Zuo-sheng YU Xing-long ZHANG Mao-lin QIAO Hong-wei CHENG Cong-sheng XU Xing-ran HU Rong-liang TU Chang-chun |
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| Institution: | LI Zuo-sheng,YU Xing-long,ZHANG Mao-lin,QIAO Hong-wei,CHENG Cong-sheng,XU Xing-ran,HU Rong-liang,TU Chang-chun~* |
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| Abstract: | The transformation/transfection efficiency of different topology plasmids for E.coli competence cell/SK cell line indicated the supercoiled plasmid DNA(closed circular DNA,cccDNA) was higher than that of the nicked DNA and linearieed DNA.The fact that the proportion of supercoiled plasmid was closely related to preparation method was also demonstrated in this study.By detecting the humoral immune response against CSFV in mice induced by the same plasmid with different topology(prepared with different methods) administered intramuscularly,it was found that the induced antibody level was positively correlated with the proportion of supercoiled plasmid.This result indicated that the topology of plasmid exerted a significant influence on DNA-mediated humoral immune response. |
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| Keywords: | plasmid DNA topology swine fever virus gene vaccine biologic property |
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