| 微卫星多重PCR分析食蚊鱼系统发育关系 |
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| 引用本文： | 刘世英,宋春高,何只翰,卢育军,吴海生,王波,曾媛琴.微卫星多重PCR分析食蚊鱼系统发育关系[J].水产学报,2017,41(10):1512-1520. |
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| 作者姓名： | 刘世英 宋春高 何只翰 卢育军 吴海生 王波 曾媛琴 |
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| 作者单位： | 1. 西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆 400715;2. 西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆 400715;昆明市第十四中学,云南昆明 650106;3. 西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆 400715;贵阳市第二实验中学,贵州贵阳 550004;4. 西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆 400715;甘肃省秦安县第一中学,甘肃天水 741600;5. 西南大学生命科学学院,淡水鱼类资源与生殖发育教育部重点实验室,重庆 400715;江西省临川第二中学,江西抚州 344000 |
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| 基金项目： | 西南大学博士基金（swu110064） |
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| 摘 要： | 食蚊鱼是全球入侵性物种之一。探讨和研究食蚊鱼快速入侵的原因对进一步的生态学研究有重要意义。实验选取11对多态性微卫星位点,检测了来自欧洲境内3条河流流经的10个不同城市53个家系的755个幼鱼样本。利用微卫星多重PCR技术及Colony统计学软件,分析食蚊鱼系谱关系。食蚊鱼的繁殖能力强,繁殖期的雌性食蚊鱼可一胎产下大量雌雄幼鱼,平均9尾,最多可达72尾;更重要的是实验中发现食蚊鱼存在多重授精现象。结果显示,来自同一父本的幼鱼胚胎最多可达41尾;多重授精加快了食蚊鱼的基因流;系统发育树证实,食蚊鱼来源于遥远的海洋,实验采集的10个群体在系统发育树中主要聚为3支。食蚊鱼入侵性强的关键原因是多重授精,强壮精子具有更强的竞争受精能力,增加了物种的多样性,但受精频率与雌鱼大小可能不成比例。在长距离的迁移中,基因流也在快速变化。
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| 关 键 词： | 食蚊鱼 多重PCR SSR 多重授精 亲子关系评估 |
| 收稿时间： | 2016/10/24 0:00:00 |
| 修稿时间： | 2017/3/14 0:00:00 |
Microsatellite SSR multiplexed amplification to analyze the phylogenetic relationship among invasive mosquitofish (Gambusia affinis) |
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| LIU Shiying,SONG Chungao,HE Zhihan,LU Yujun,WU Haisheng,WANG Bo and ZENG Yuanqin.Microsatellite SSR multiplexed amplification to analyze the phylogenetic relationship among invasive mosquitofish (Gambusia affinis)[J].Journal of Fisheries of China,2017,41(10):1512-1520. |
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| Authors: | LIU Shiying SONG Chungao HE Zhihan LU Yujun WU Haisheng WANG Bo and ZENG Yuanqin |
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| Institution: | Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Science in Southwest University, Chongqing 400715, China,Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Science in Southwest University, Chongqing 400715, China;The No. 14 Middle School of Kunming, Kunming 650106, China,Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Science in Southwest University, Chongqing 400715, China;Guiyang No.2 Experimental Middle School, Guiyang 550004, China,Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Science in Southwest University, Chongqing 400715, China;Qin''an No.1 Middle School of Gansu Province, Tianshui 741600, China,Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Science in Southwest University, Chongqing 400715, China;Linchuan No.2 Middle School of Jiangxi Province, Fuzhou 344000, China,Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Science in Southwest University, Chongqing 400715, China and Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, School of Life Science in Southwest University, Chongqing 400715, China |
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| Abstract: | Mosquitofish(Gambusia affinis)is a globe invasive species. In order to analyze why they can invade so quickly all over the world, 11 polymorphic microsatellite loci were isolated and employed to examine paternity patterns in 755 samples of 53 families from 10 locations in 3 rivers basins. After sequence genetic analysis of the multiplex SSR PCRs, we got the best fullsib family and analyzed the phylogenetic relationship. We found that there are multiple inseminations in G. affinis. The female in the same womb can reproduce a number of the juveniles, up to 72, with different males, even up to 9. The max fullsib is 41, but the breeder''s size isn''t the max even not bigger than others. At the same time the max size of the breeder isn''t the female which reproduce the largest number of the juveniles. The phylogenetic tree shows that the evolution was far away the sea. The 10 populations can be obviously divided into 3 clusters. The conclusion can be drawn that the key reason of the invasion is G. affinis multiple inseminations. It is suspected that the fertilized frequency isn''t proportional to the size of female. Their gene flows rapidly specifically when they were in the migratory distance. |
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| Keywords: | Gambusia affinis|multiplex PCR|SSR|multiple insemination|parentage assessment |
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