| 西藏普通小麦高分子量谷蛋白亚基多态性分析 |
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| 引用本文： | 代寿芬,颜泽洪,魏育明,郑有良.西藏普通小麦高分子量谷蛋白亚基多态性分析[J].西北农业学报,2004,13(2):90-93,100. |
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| 作者姓名： | 代寿芬 颜泽洪 魏育明 郑有良 |
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| 作者单位： | 四川农业大学小麦研究所,都江堰,611830 |
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| 基金项目： | 国家自然科学基金(30370882)，四川省教育厅自然科学基金预研项目。 |
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| 摘 要： | 采用十二烷基磺酸钠一聚丙烯酰胺凝胶电泳(SDS—PAGE)法，对229份西藏普通小麦高分子量谷蛋白亚基组成进行了鉴定和分析。研究表明，229份西藏普通小麦共检测到19种亚基组合类型，其中以null，7 8，2 12组合类型居多。在各位点的等位变异中，Glu—B1位点出现的等位变异最多，共9种；Glu—D1和Glu—A1位点均出现4种等位变异。Glu—A1和Glu—B1位点分别检测到一个的新亚基，暂定名为5#和6#。品质评分表明，西藏普通小麦的品质评分为4～10分，多数品质评分在6分及6分以下，同时筛选到2份(As1531 and As1772)评分达10分的材料。
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| 关 键 词： | 西藏 普通小麦 高分子量谷蛋白亚基 多态性 SDS-PAGE 品质 |
| 文章编号： | 1004-1389(2004)02-0090-04 |
| 收稿时间： | 2003/9/18 0:00:00 |
| 修稿时间： | 2004/2/16 0:00:00 |
Allelic Variations of High Molecular Weight Glutenin Subunits (HMW-GS) in Tibetan Wheat |
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| DAI Shou-fen,YAN Ze-hong,WEI Yu-ming and ZHENG You-liang.Allelic Variations of High Molecular Weight Glutenin Subunits (HMW-GS) in Tibetan Wheat[J].Acta Agriculturae Boreali-occidentalis Sinica,2004,13(2):90-93,100. |
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| Authors: | DAI Shou-fen YAN Ze-hong WEI Yu-ming and ZHENG You-liang |
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| Institution: | Triticeae Research Institute of Sichuan Agricultural University, Dujiangyan 611830, China;Triticeae Research Institute of Sichuan Agricultural University, Dujiangyan 611830, China;Triticeae Research Institute of Sichuan Agricultural University, Dujiangyan 611830, China;Triticeae Research Institute of Sichuan Agricultural University, Dujiangyan 611830, China |
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| Abstract: | Allelic variation of high molecular weight glutenin subunits (HMW-GS) was investigated by using one-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis from 229 accessions of Tibetan wheat. 19 subunits combinations were found, among which, combinations null, 7 8 and 2 12 was the most frequent combination. Furthermore, two novel high-molecular-weight glutenin subunits, tentative named 5~* and 6~*, were detected in Tibetan wheat. The quality score of each Tibetan wheat collection varied from 4 to 10. The quality scores of 177 accessions (83.9%) were 6 or below, while there had 2 accessions (i.e. As1531 and As1772) with the highest score (i.e. 10). |
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| Keywords: | Tibetan wheat High Molecular Weight Glutenin Subunits (HMW-GS) SDS-PAGE Quality score |
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