| 固始鸡免疫器官内细胞凋亡基因Fas和FasL的动态表达 |
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| 引用本文： | 李奎,康相涛,刘英,孙桂荣.固始鸡免疫器官内细胞凋亡基因Fas和FasL的动态表达[J].中国农业科学,2008,41(5):1489-1496. |
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| 作者姓名： | 李奎 康相涛 刘英 孙桂荣 |
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| 作者单位： | [1]河南农业大学牧医工程学院,郑州450002 [2]甘肃农业大学动物医学院,兰州730070 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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国家科技支撑计划
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公益性行业(农业)科研专项
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河南省高校杰出科研创新人才工程项目
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河南省教育厅自然科学基金 |
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| 摘 要： | 【目的】探讨固始鸡免疫器官内细胞凋亡基因Fas和FasL的动态表达。【方法】应用免疫组织化学技术和Leica 显微图像处理系统，对细胞凋亡基因Fas和FasL在固始鸡免疫器官内的动态表达进行研究。【结果】Fas在不同发育阶段固始鸡免疫器官内均有表达，但表达量均不相同，呈波浪样动态变化；Fas表达于固始鸡免疫器官内淋巴细胞细胞膜和细胞质，不表达于细胞核；Fas表达阳性细胞在固始鸡不同免疫器官内分布位置不同，呈散在或簇团状分布：法氏囊内阳性细胞主要分布于黏膜靠近上皮细胞的固有膜层内、淋巴小结与淋巴小结之间区域、淋巴小结边缘，淋巴小结内少量淋巴细胞也有Fas表达；胸腺内主要分布于胸腺小叶髓质，极少出现在皮质内；脾脏内主要分布于红髓和边缘区、淋巴小结周围和动脉周围淋巴鞘周围的区域，动脉周围淋巴鞘极少有Fas表达，淋巴小结无Fas表达。FasL在固始鸡免疫器官内的表达与Fas相似，但表达量与Fas相比较少。【结论】细胞凋亡基因Fas和FasL参与了固始鸡免疫器官内淋巴细胞发育分化过程中的凋亡调控，并对免疫器官的稳定发挥重要作用。
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| 关 键 词： | 细胞凋亡 Fas FasL 凋亡基因表达 免疫组织化学 免疫器官 固始鸡 |
| 收稿时间： | 2007-1-12 |
| 修稿时间： | 2007年1月16日 |
Dynamic Expression of Apoptosis Gene Fas and FasL in Immunity Organs of Gushi Chickens |
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| LI Kui,KANG Xiang-tao,LIU Ying,SUN Gui-rong.Dynamic Expression of Apoptosis Gene Fas and FasL in Immunity Organs of Gushi Chickens[J].Scientia Agricultura Sinica,2008,41(5):1489-1496. |
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| Authors: | LI Kui KANG Xiang-tao LIU Ying SUN Gui-rong |
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| Abstract: | Abstract: The dynamic expression of the apoptotic gene-Fas/FasL in immunity organs of Gushi chickens was studied by using immunohistochemistry technology and Leica Microsystems, and its physiological significane was disscussed. The results showed that the expression of Fas in different immunity organs were observed, however, the number of the positive Fas-cells were different between different developmental stages. The positive Fas-protein was found in cell membrane, cytoplasm, but not in nucleus of lymphocytes. The positive Fas-cells were dispersed (or in clusters), and distributed in different places for different immunity organs. The majority of positive Fas-cells, in bursa, were observed in lamina propria of mucous membrane near epithelium and areas between lymphoid nodules, edges of lymphoid nodules, and a few in inner of lymphoid nodules. In thymus, the positive Fas-cells localized mainly in thymic medulla, and few in thymic cortex. The positive Fas-cells, in spleen, were found predominantly in red pulp, marginal zones, and the surronding areas of splenic nodules and periarterial lymphatic sheath, few in splenic nodules and periarterial lymphatic sheath. The expression of FasL in immunity organs of Gushi chickens is similar to Fas, but, the positive FasL-cells is less than Fas. All results mentioned above indicate that the apoptotic gene-Fas/FasL participates in the regulation of apoptosis of thymocytes and lymphocytes in immunity organs of Gushi chickens, and it is important for the stability of immunity organs. |
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| Keywords: | Expression of apoptotic gene FasL Fas Apoptosis Gushi chicken Immunity organ Immunohistochemistry Expression of apoptotic gene FasL Fas Apoptosis Fas FasL Expression of apoptotic gene Immunohistochemistry Immunity organ Gushi chicken Apoptosis |
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