| 麻竹叶抑菌活性成分的分离鉴定 |
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| 引用本文： | 谢碧霞,陆志科.麻竹叶抑菌活性成分的分离鉴定[J].中南林业科技大学学报,2005,25(5):10-14. |
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| 作者姓名： | 谢碧霞 陆志科 |
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| 作者单位： | 中南林学院，中南林学院 湖南 株洲 412006，湖南 株洲 412006 广西大学林学院，广西 南宁 530001 天津大学药物科学与技术学院，天津 530005 |
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| 基金项目： | 中南林学院校科研和教改项目 |
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| 摘 要： | 对麻竹叶抑菌活性成分进行了分离鉴定.结果表明:将麻竹叶干粉加70%乙醇抽提至色淡,抽提液浓缩后经石油醚萃取,水相过聚酰胺柱,用水和70%乙醇洗脱,醇洗部回收溶剂经60℃低温干燥,得红棕色结晶性粉末;该物质经真空层析、聚酰胺层析,得单一色斑;在聚酰胺柱色谱中呈单一吸收蜂,经高压液相色谱检验,单一主峰面积比例为97.75%;用盐酸镁粉检出其为黄酮化合物;经酸水解,用硅胶薄板层析检出有木糖;用UV-vis光谱法及化学法对其结构进行分析,其分子结构为黄酮醇类,该化合物具有较强抑菌活性,酸水解后抑菌活性升高.
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| 关 键 词： | 经济林 麻竹叶 纯化 分子结构 抑菌作用 |
| 文章编号： | 1000-2502(2005)05-0010-05 |
| 修稿时间： | 2005年1月12日 |
The Separation and Identification of Antimicrobial Active Substances in Sinocalamus latiflorus McClure leaves |
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| XIE Bi-xia,LU Zhi-ke.The Separation and Identification of Antimicrobial Active Substances in Sinocalamus latiflorus McClure leaves[J].Journal of Central South Forestry University,2005,25(5):10-14. |
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| Authors: | XIE Bi-xia LU Zhi-ke |
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| Abstract: | The extract of Sinocalamus latiflorus McClure leaves has lots of bioaetivities. This paper introduces in detail methods of extraction, separation, purifition and analyzes the structure of the extract. The result proved by HPLC shows that the extract by 70% ethanol is a simple substance (97.75%) after its purification by vacuum and polyamide chromatographer. It has also been proved by Uv-vis and chemistry that the extract is a flavonoid and its molecular structure is flavonoids. The extract has a higher antimicrobial effect after it is hydrolyzed. |
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| Keywords: | nonwood forest Sinocalamus latiflorus McClure leaves purification molecular structure antimicrobial action |
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