| 多浆旱生植物霸王质膜Na~＋/H~＋逆向转运蛋白基因RNAi载体构建 |
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| 引用本文： | 马清,王锁民.多浆旱生植物霸王质膜Na~＋/H~＋逆向转运蛋白基因RNAi载体构建[J].草业科学,2012,29(4):549-553. |
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| 作者姓名： | 马清 王锁民 |
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| 作者单位： | 草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州,730020;草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州,730020 |
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| 基金项目： | 国家自然科学基金(31072073);教育部博士学科点专项科研基金(20090211110001) |
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| 摘 要： | 以多浆旱生植物霸王(Zygophyllum xanthoxylum)幼苗根系为材料，采用RT PCR方法获得了其质膜Na+/H+逆向转运蛋白基因ZxSOS1的片段，以中间载体pHANNIBAL和植物表达载体PART27为基础，采用酶切连接的方法构建了CaMV 35S启动子驱动的含ZxSOS1基因片段反向重复序列的RNAi植物表达载体PARS，为利用RNAi技术深入研究ZxSOS1在霸王体内Na+转运中的功能及其在霸王适应干旱生境中的作用机制奠定基础。
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| 关 键 词： | 霸王 ZxSOS1 RNAi载体构建 |
Construction of RNAi expression vector of the plasma membrane Na~+/H~+ antiporter gene in succulent xerophyte Zygophyllum xanthoxylum |
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| MA Qing,WANG Suo-min.Construction of RNAi expression vector of the plasma membrane Na~+/H~+ antiporter gene in succulent xerophyte Zygophyllum xanthoxylum[J].Pratacultural Science,2012,29(4):549-553. |
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| Authors: | MA Qing WANG Suo-min |
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| Institution: | (State Key Laboratory of Grassland Agro-ecosystems;College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,China) |
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| Abstract: | Total RNA was extracted from roots of succulent xerophyte Zygophyllum xanthoxylum seedlings,and the fragment of the plasma membrane Na+/H+ antiporter gene ZxSOS1 was amplified by RT-PCR.Then the RNAi plant expression vector PARS with inverted repeats of ZxSOS1 driven by the promoter CaMV 35S was constructed based on the intermediate vector pHANNIBAL and the plant expression vector PART27 by restriction enzyme digestion and ligation.The work might be a good basis for further investigation of the roles of ZxSOS1 in the transportation of Na+ and the adaption to arid environment in Z.xanthoxylum by RNAi technique. |
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| Keywords: | Zygophyllum xanthoxylum ZxSOS1 construction of RNAi plant expression vector |
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