| 热激处理对枇杷2n花粉发生率的影响 |
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| 引用本文： | 汪卫星,郭启高,向素琼,李晓林,梁国鲁.热激处理对枇杷2n花粉发生率的影响[J].果树学报,2003,20(4):284-286. |
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| 作者姓名： | 汪卫星 郭启高 向素琼 李晓林 梁国鲁 |
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| 作者单位： | 西南农业大学园艺园林学院,重庆,北碚,400716 |
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| 基金项目： | 国家自然科学基金(批准号:30170656)，国家教育部“高等学校骨干教师资助计划”项目(批文号:教技司2000) |
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| 摘 要： | 以龙泉1号枇杷为试材,用热激处理诱导小孢子2n配子的发生;并通过观察天然四倍体枇杷花粉,研究其中正常花粉与2n花粉在形态上的异同及其所占的比例。结果表明:30℃/12 h的处理可以使枇杷2n花粉的发生率由对照的0.26％提高到2.64％,败育率约为2.44％。32℃/12 h处理过后的枇杷花粉由于败育率达到20％以上,不能准确计算2n花粉所占比例;在天然四倍体枇杷花粉中存在四种直径不同的花粉,即45μm左右的特大花粉、31μm左右的大花粉。25μm左右的中型花粉和18μm左右的败育花粉,其比例分别为0.1％、54.52％、19.32％和26.06％。
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| 关 键 词： | 枇杷 热激处理 2n配子 |
| 文章编号： | 1009-9980(2003)04-284-03 |
| 修稿时间： | 2002年11月30 |
Study on the Effect of Hot-shock Treatment on the Occurrence Frequency of 2n Pollen of Loquat Trees |
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| Wang Weixing,Guo Qigao,Xiang Suqiong,Li Xiaolin,and Liang Guolu.Study on the Effect of Hot-shock Treatment on the Occurrence Frequency of 2n Pollen of Loquat Trees[J].Journal of Fruit Science,2003,20(4):284-286. |
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| Authors: | Wang Weixing Guo Qigao Xiang Suqiong Li Xiaolin and Liang Guolu |
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| Abstract: | Longquan No. 1, a loquat variety was used as material for studying the effect of hot-shock on the occurrence frequency of 2n pollen. Microspore with 2n gametes were induced by hot-shock treatment, then the morphologism and proportion of 2n pollen were compared with natural tetraploidy loquat (Eriobotryajaponica Lindl.). The results showed: (1) The 2n pollen frequency increased from 0.26%(the control) to 2.64% induced by the treatment of hot-shock of 30℃/12 h, and the abortion percent was about 2.44%; furthermore the abortion percent was over 20% induced by the treatment of 32℃/12 h, yet the proportion of natural 2n pollen could not be accurately calculated. (2) There were four categories of the diameter of pollens in the natural tetraploid loquat: ±45 μ,m super large pollen, ±31 μm large pollen, ±25 μm middle pollen and ±18 μm abortion pollen, the frequency was 0.1 %, 54.52%, 19.32%, 26.06%, respectively. |
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| Keywords: | Eriobotryajaponica Lindl Loquat Hot-shock treatment 2n gamete |
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