| 欧洲野生甘蓝的核质遗传多样性和群体遗传结构分析 |
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| 引用本文： | 许鲲,陆光远,伍晓明,高桂珍,陈碧云,吕培军.欧洲野生甘蓝的核质遗传多样性和群体遗传结构分析[J].中国油料作物学报,2011,33(2):111-117. |
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| 作者姓名： | 许鲲 陆光远 伍晓明 高桂珍 陈碧云 吕培军 |
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| 作者单位： | 农业部油料作物生物学重点开放实验室，中国农科院油料作物研究所，湖北 武汉 430062
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| 基金项目： | 国家863计划，动植物品种分子设计专题，国家自然科学基金 |
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| 摘 要： | 为了解欧洲野生甘蓝遗传背景，以栽培甘蓝、甘蓝型油菜和白菜型油菜作对比，对引进的7个生态地理群体共80份野生甘蓝材料进行了核质遗传多样性和群体遗传结构分析。利用10对特异性SSR引物分析叶绿体基因组多样性，获得8个差异性标记，将参试94份材料划分为3类，即甘蓝（含栽培和野生甘蓝）、甘蓝型油菜和白菜型油菜；包括6种单倍型，其中野生甘蓝2个，甘蓝型油菜3个，白菜型油菜1个。利用6对SRAP引物对核基因组多样性进行分析，获得75个多态性标记；聚类及遗传结构分析表明7个野生甘蓝群体中，F、D、G三个群体聚为独立的亚类，E群体大部分单株归于G亚类。其它3个群体A、B、C则相互混杂在一起。野生甘蓝群体Nei’s基因多样性指数（H）和Shannon’s 指数（I）分别为0.301 3和0.461 1。分子方差分析(AMOVA)表明野生甘蓝以群体内变异为主，占80%；群体间变异仅占20%。结果显示引进的野生甘蓝群体核质遗传多样性丰富。
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| 关 键 词： | 野生甘蓝 遗传多样性 群体遗传结构 |
Nuclear - cytoplasmic diversity and population structure of European wild Brassica oleracea |
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| XU Kun,LU Guang-yuan,WU Xiao-ming,GAO Gui-Zhen,CHEN Bi-yun,LV Pei-jun.Nuclear - cytoplasmic diversity and population structure of European wild Brassica oleracea[J].Chinese Journal of Oil Crop Sciences,2011,33(2):111-117. |
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| Authors: | XU Kun LU Guang-yuan WU Xiao-ming GAO Gui-Zhen CHEN Bi-yun LV Pei-jun |
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| Institution: | XU Kun,LU Guang-yuan,WU Xiao-ming*,GAO Gui-Zhen,CHEN Bi-yun,LV Pei-jun (Key Laboratory of Oil Crop Biology of the Ministry of Agriculture,Oil Crops Research Institute,CAAS,Wuhan 430062,China) |
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| Abstract: | To understand the genetic diversity and population structure of wild Brassica oleracea,80 accessions of European B.oleracea in 7 geographic populations were analyzed.14 representative materials as cultivated B.oleracea,B.napus and B.rapa were used as references.Chloroplast DNA was examined using 10 specific SSR primers which generated 8 polymorphic bands.According to these bands and markers,94 materials were divided into 3 groups as B.oleracea(cultivated and wild ones),B.napus and B.rapa.They were also divi... |
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| Keywords: | Wild Brassica oleracea Genetic diversity Population genetic structure |
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