| 应用聚合酶链反应检测鉴别火鸡支原体方法的建立 |
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| 引用本文： | 谢芝勋,邓显文,谢志勤.应用聚合酶链反应检测鉴别火鸡支原体方法的建立[J].中国预防兽医学报,2000,22(3):215-217. |
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| 作者姓名： | 谢芝勋 邓显文 谢志勤 |
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| 作者单位： | 广西兽医研究所,南宁 |
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| 基金项目： | 广西留学回国人员基金，国家非教育系统留学回国人员择优项目资助 |
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| 摘 要： | 根据基因库中火鸡支原体（ＭＭ）１６Ｓ ｒＲＮＡ基因序列（Ｇｅｎｅｂａｎｋ Ｕ０４６４９），设计了一对跨幅为８５０ｂｐ的引物，用这对引物对４禽侏火鸡支原体标准菌株和６种其它禽病病原体进行ＰＣＲ扩增，结果４禽株鸡支原体菌株均得到片段大小与预期设计相一致的８５０ｂｐ的ＰＣＲ扩增产物，而其它６种禽病病原体（包括ＭＧ、ＭＳ和ＭＩ）的扩增结果则均为阴性，该ＰＣＲ能检出１００ｆｇ的火鸡支原体的ＤＮＡ模板。
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| 关 键 词： | 火鸡支原体 聚合酶链反应 检测 鉴别 16S rRNA |
| 修稿时间： | 1999-11-25 |
Detection and Identification of Mycoplasma Meleagridis by Polymerase Chain Reaction |
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| XIE Zhixun,DENG Xianweng,XIE Zhiqin,PANG Yaoshang,LIU Jiabo.Detection and Identification of Mycoplasma Meleagridis by Polymerase Chain Reaction[J].Chinese Journal of Preventive Veterinary Medicine,2000,22(3):215-217. |
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| Authors: | XIE Zhixun DENG Xianweng XIE Zhiqin PANG Yaoshang LIU Jiabo |
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| Abstract: | A set of primers,XZ19、XZ20 were designed accord
ing to the&127;sequence of 16s rRNA gene of Mycoplasma Meleagridis (MM) from Genebank
U04649. A MM specific 850-base pair DNA product was amplified by thes e&127;primers
from four MM reference strains,but not from six other avian pathogenic virus and bacteria
(including MG、MS and MM). As little as 100fg of&127;MM DNA was detected using gel
electrophroesis. |
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| Keywords: | Mycoplasma Meleagridis Polymerase Chain Reaction Detection |
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