| 喹乙醇对鲤的消化酶活性及白细胞吞噬功能的影响 |
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| 引用本文： | 叶继丹,卢彤岩,刘红柏,赵吉伟,杨雨辉.喹乙醇对鲤的消化酶活性及白细胞吞噬功能的影响[J].大连水产学院学报,2004,19(3):161-166. |
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| 作者姓名： | 叶继丹 卢彤岩 刘红柏 赵吉伟 杨雨辉 |
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| 作者单位： | 中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070;中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070;中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070;中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070;中国水产科学研究院,黑龙江水产研究所,黑龙江,哈尔滨,150070 |
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| 基金项目： | 黑龙江省科委攻关(GZD3B110)，中国水产科学研究院人才基金资助(2000-B-11) |
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| 摘 要： | 对7组1龄鲤Cyprinus carpio L分别饲喂添加不同剂量的喹乙醇饲料(0、100、200、400、800、1600、3200mg／kg)。于试验的第3、6、9、12周末，从每组随机抽取4～5尾鱼采取血样，测定肝胰脏及肠道中蛋白酶、淀粉酶、脂肪酶活性以及血液白细胞吞噬指数、吞噬率、头肾／体重等指标，评价喹乙醇对鲤消化能力和非特异性免疫功能的影响。结果表明，当喹乙醇的添加剂量为100～800mg／kg时，鲤蛋白酶活性被诱导；剂量为1600～3200mg／kg时，鲤蛋白酶活性则被抑制。淀粉酶活性在不同喹乙醇剂量下被明显诱导。试验前期，中低剂量的喹乙醇可诱导脂肪酶活性，而较高剂量却抑制其活性；试验后期，喹乙醇对鲤脂肪酶活性的影响不明显。喹乙醇对鲤血液白细胞吞噬指数、吞噬率及头肾体重比没有明显的影响。这说明喹乙醇对鲤消化酶活性的影响较大，而对非特异性免疫功能及免疫器官的发育则没有影响。
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| 关 键 词： | 鲤 喹乙醇 消化酶活性 吞噬活性 |
| 文章编号： | 1000-9957(2004)03-0161-06 |
Effect of olaquindox on digestive enzyme activities and phagocytosis of leucocytes in Cyprinus carpio L. |
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| YE Ji-dan,LU Tong-yan,LIU Hong-bai,ZHAO Ji-wei,YANG Yu-hui.Effect of olaquindox on digestive enzyme activities and phagocytosis of leucocytes in Cyprinus carpio L.[J].Journal of Dalian Fisheries University,2004,19(3):161-166. |
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| Authors: | YE Ji-dan LU Tong-yan LIU Hong-bai ZHAO Ji-wei YANG Yu-hui |
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| Abstract: | A feeding trial with one-year old Cyprinus carpio L. was conducted to study the influence of dietary olaquindox (0, 100, 200, 400, 800, 1600, 3200 mg/kg) on activities of protease, amylase and lipase, phagocytic index and phagocytic rate of leucocytes and ratio of head kidney weight to body weight. Bloods were sampled from 4-5 fish in each treatment and determined for the blood values at the end of week 3, week 6, week 9 and week 12. The results showed that protease activity was induced at a dose of 100 mg/kg feed of olaquindox, but suppressed over the dose of 1600 mg/kg feed. Amylase activity was elevated with olaquindox treated groups. Lipase activity increased at low-mid doses of olaquindox in the early period of the experiment, but decreased at high dosages of olaquindox in the later period of the experiment. There were no significant difference in phagocytic index and phagocytic rate of leucocytes and ratio of head kidney weight to body weight between olaquindox-treated groups and the control group, suggesting that olaquindox can affect markedly digestive enzyme activities, and that does not have impacts non-specific immunological function and growth of immune organ in common carp. |
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| Keywords: | Cyprinus carpio L olaquindox digestive enzyme activity phagocytosis |
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