| 拮抗细菌Bacillus subtilis A30对水稻病原菌的抑制作用 |
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| 引用本文： | 陈卫良,龚鸿飞,林福呈,许建平,李德葆.拮抗细菌Bacillus subtilis A30对水稻病原菌的抑制作用[J].浙江大学学报(农业与生命科学版),1997(6). |
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| 作者姓名： | 陈卫良 龚鸿飞 林福呈 许建平 李德葆 |
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| 作者单位： | 浙江农业大学生物技术研究所 |
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| 摘 要： | 由杭州郊区水稻叶围分离得到ＢａｃｉｌｕｓｓｕｂｔｉｌｉｓＡ３０菌株，平板检测表明它对Ｘａｎ－ｔｈｏｍｏｎａｓｏｒｙｚａｅｐｖ．ｏｒｙｚａｅ、Ｒｈｉｚｏｃｔｏｎｉａｓｏｌａｎｉ、Ｆｕｓａｒｉｕｍｍｏｎｉｌｉｆｏｒｍｅ和Ｍａｇｎａｐｏｒｔｈｅｇｒｉｓｅａ等多种水稻病原真菌、病原细菌均有很好的拮抗作用．以水稻白叶枯病、水稻纹枯病为代表进行小区试验，结果表明，Ａ３０菌株的防病效果分别为５８．１％和３９．０％．Ａ３０菌株的培养去菌液经７０％饱和度（ＮＨ４）２ＳＯ４处理，拮抗物可被（ＮＨ４）２ＳＯ４沉淀，其拮抗粗提液经Ｄｉｏｌ１５０及ｐｒｏＲＰＣＨＲ５／１０等色谱柱分离得到两种对Ｘ．ｏｒｙｚａｅｐｖ．ｏｒｙｚａｅ的拮抗峰和两种对Ｒ．ｓｏｌａｎｉ的拮抗峰．
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| 关 键 词： | Bacillus subtilis A30 拮抗作用 拮抗物质 水稻病原菌 |
The antagonistic activities of Bacillus subtilis A30 to rice pathogens |
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| Chen Weiliang, Gong Hongfei, Lin Fuchen, Xu Jianping, Li Debao.The antagonistic activities of Bacillus subtilis A30 to rice pathogens[J].Journal of Zhejiang University(Agriculture & Life Sciences),1997(6). |
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| Authors: | Chen Weiliang Gong Hongfei Lin Fuchen Xu Jianping Li Debao |
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| Abstract: | Bacillus subtilis A30 was isolated from the phyllosphere of rice in the suburb of Hangzhou In vitro tests, it showed antagonistic activities against Xanthomonas oryzae pv oryzae, Rhizoctonia solani, Magnaporthe grisea and Fusarium monilifome which are all the rice pathogens In the plot experiments, the A30 strain could inhibit the development of the rice bacterial leaf blight and the rice sheath blight with effect of 58 1% and 39 0%, respectively The antagonistic substances from the B subtilis A30 extract could be precipitated by 70% saturated (NH 4) 2SO 4 and were isolated and purified partially by chromatography with Diol 150 columm and proRPC HR5/10 column The results showed two antagonistic peaks to X oryzae pv oryzae and two antagonistic peaks to R solani |
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| Keywords: | Bacillus subtilis A30 antagonistic activities antagonistic substances rice pathogens |
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