Nutrient starvation affects expression of LC3 family at the feto-maternal
interface during murine placentation |
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| Authors: | Masato HIYAMA Ken Takeshi KUSAKABE Ai TAKESHITA Sinji SUGI Nobue KUNIYOSHI Hiroyuki IMAI Kiyoshi KANO Yasuo KISO |
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| Institution: | 1)Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753–8515, Japan;2)Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753–8515, Japan;3)Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Science, Osaka Prefecture University, Osaka 598–8531, Japan |
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| Abstract: | LC3 − the mammalian homolog of Atg8 − was found as autophagosome membrane binding protein
in mammals and widely used as an autophagosomal marker. LC3A, B and C show different
expression patterns in each tissue. The aim of this study was to reveal the differences of
expression patterns among LC3 families in mouse placenta under normal condition and
nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and
B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was
decreased. Starvation induced increase in LC3B expression specifically.
Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A
expression in syncytiotrophoblast was vanished by starvation. The results of real time
RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by
starvation. Taken together, this study suggests that autophagy could play a role in
placental invasion system and that nutrient starvation affects LC3B expression. |
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| Keywords: | autophagy LC3 mTOR placenta starvation |
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