| HPLC法测定玉米酒糟中绿原酸的含量 |
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| 引用本文： | 孙健,刘嘉琦,李显,孙召伟,李俊婷,常桂英,于加平.HPLC法测定玉米酒糟中绿原酸的含量[J].吉林农业科技学院学报,2013(3):12-14. |
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| 作者姓名： | 孙健 刘嘉琦 李显 孙召伟 李俊婷 常桂英 于加平 |
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| 作者单位： | 吉林农业科技学院生物工程学院,吉林132101 |
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| 基金项目： | 吉林省科技厅项目（201215186）;吉林农业科技学院大学生创新科研项目 |
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| 摘 要： | 采用浸提法提取玉米酒糟中绿原酸并用HPLC法测定其中的含量,采用安捷伦色C18（4.6mm×150mm,5μm）色谱柱,乙腈-0.5％磷酸溶液为流动相,流速为1.0mL·min-1,检测波长为327nm.结果表明绿原酸在0.232～2.784μg呈现良好的线性关系,玉米酒糟中绿原酸含量0.028％,平均回收率为95.80％,RSD=2.72％.试验证明了玉米酒糟中含有绿原酸,且其含量较高,为玉米酒糟的进一步开发提供科学依据.
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| 关 键 词： | 玉米酒糟 绿原酸 高效液相色谱 |
Using HPLC Method to Determine the Content of Chlorogenic Acid in Corn Distillers |
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| SUN Jian,LIU Jiaqi,LI Xian,SUN Zhaowei,LI Junting,CHANG Guiying,YU Jiaping.Using HPLC Method to Determine the Content of Chlorogenic Acid in Corn Distillers[J].Journal Of Jilin Agricultural Science And Technology College,2013(3):12-14. |
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| Authors: | SUN Jian LIU Jiaqi LI Xian SUN Zhaowei LI Junting CHANG Guiying YU Jiaping |
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| Institution: | ( Jilin Agricultural Science and Technology University School of Biology Engineering, Jilin 132101) |
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| Abstract: | Using extraction method to extract the Chlorogenic acid in corn distillers and using HPLC method to determine the content in it, the column was Agilent color C18（4.6mm × 150mm,5μm）, Acetonitrile- 0.5% phos- phoric acid was the mobile phase, the mobile speed was 1.0mL·min-1, and the detection wavelength was 327nm. The results showed that the Chlorogenic acid showed a good linear relationship in 0.232 - 2.784μg, the content was 0.028%, the average recovery was 95.80% ,RSD= 2.72%. The test showed that there were Chlorogenic acid in corn distillers, and the contents were higher, and provided the scientific basis for further development of corn dis- tillers. |
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| Keywords: | corn distillers Chlorogenic acid HPLC |
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