| 神灯白掌组织培养的研究 |
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| 引用本文： | 吴丽君.神灯白掌组织培养的研究[J].福建林学院学报,2001,21(2):169-172. |
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| 作者姓名： | 吴丽君 |
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| 作者单位： | 福建省林业科技试验中心, |
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| 摘 要： | 以 MS为基本培养基 ,在不同激素成份及浓度水平下 ,诱导神灯白掌茎尖 ,进行组培试验 .研究表明 :适合茎尖诱导培养基为改良 MS+ 6 - BA1 .5～ 3 .0 mg·L-1+ NAA0 .5～ 1 .0 mg·L-1,诱导率为 90 % ;适合不定芽增殖培养基为改良 MS+ 6 - BA 0 .5～ 2 .5mg· L-1+ NAA 0 .5～1 .5mg· L-1,芽年增殖系数达 4.2 1 2 ,适合生根诱导培养基为 1 /2 MS+ NAA 1 .0 mg· L-1或 1 /2 MS+ IBA1 .0 mg· L-1,生根率可达 90 %以上 .选择继代一级芽苗直接移植于基质中 ,进行瓶外发根培养 ,成活率可达 85%以上 ,是加快工厂化育苗速度及降低组培苗成本的有效途径
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| 关 键 词： | 神灯白掌 组织培养 快速繁殖 瓶外发根 |
| 文章编号： | 1001-389X(2001)02-0169-04 |
| 修稿时间： | 2000年10月13 |
A Study on the Tissue Culture Spathiphyllum floribundu |
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| WU Li-jun.A Study on the Tissue Culture Spathiphyllum floribundu[J].Journal of Fujian College of Forestry,2001,21(2):169-172. |
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| Authors: | WU Li-jun |
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| Abstract: | Using the MS culture medium,the shoot tip of Spathiphyllum floribundu was induced and cultured with the different hormone in different concentration levels The results revealed that the suitable culture medium for shoot tip induction was the improved MS+6BA 1 5~3 0 mg/L+ NAA 0 5~1 0 mg/L,whose induction rate reached 90%; the suitable culture medium for adventitious bud multiplication was the improved MS+6BA 0 5~2 5 mg/L+NAA 0 5~1 5 mg/L,in which the annual multiplication coefficient of adventitious bud gained 4 212;the suitable culture for rooting induction was 1/2 MS+NAA 1 0 mg/L or 1/2MS+IBA 1 0 mg/L,whose rooting rate was more than 90% The first rate generation continuing seedling were selected,transplanted and cultured for taking root on the culture medium outside the test tube,whose survival rate reached more than 85% So,it was an effective way to quicken the factory seedling speed and to reduce the cost of tissue culture seedling |
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| Keywords: | Spathiphyllum floribundu tissue culture rapid propagation taking root outside test tube |
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