Silver nitrate and adenine sulphate induced high regeneration frequency in the recalcitrant plant Cosmos bipinnatus using cotyledon explants |
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| Authors: | Mahdi Jaberi Behzad Gharehyazi Mahmood Khosrowchahli Ali Sharafi Nafiseh Aboofazeli |
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| Institution: | 1. Department of Agricultural Biotechnology of Science and Research Branch, Islamic Azad University, Tehran, Iran;2. Novin Giti Gene R&3. D Co. (N.G.G), National Institute of Genetic Engineering &4. Biotechnology, Biotechnology Incubator Center, Tehran, Iran;5. Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran;6. Genetics Department of Plant Breeding, Faculty of Agriculture and Natural Resources, Tehran Azad University, Science and Research Branch, Tehran, Iran;7. Pharmaceutical Biotechnology Department School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran;8. Novin Giti Gene R& |
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| Abstract: | Plant regeneration ability was studied in the medicinal-ornamental plant, Cosmos bipinnatus ‘Sonata white’, which is a dicotyledonous recalcitrant plant to shoot induction. Cotyledons were used as sources of explants to investigate plant regeneration. High frequency of direct shoot induction was obtained when BA (5 mg/l) and AgNO3 (5 mg/l) were used in combination with 20 mg/l adenine sulphate (73.8%) in Murashige and Skoog (MS) medium. The highest shoot number per explant (5.7) was induced on MS medium supplemented with 5 mg/l BA, 5 mg/l AgNO3, and 40 mg/l adenine. Eight week-old shoots were transferred to root induction media containing MS and half-strength MS medium with different concentration of IBA. The highest rate of root induction (70.8%) was obtained on half-strength MS medium with 1.5 mg/l IBA within four weeks. The plantlets were transferred to pot and kept in the greenhouse condition. Seventy percent of the plantlets successfully acclimatised. Abbreviations: BA, 6-benzylaminopurine; IBA, Indole-3-butyric acid; MS, Murashige and Skoog; PGRs, plant growth regulators. |
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| Keywords: | Cosmos bipinnatus direct organogenesis silver nitrate adenine sulphate recalcitrant plant |
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