| 基于CRISPR/Cas系统的现场核酸检测技术 |
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| 引用本文： | 蒋静,赵文亮,李文越,陶大刚,潘文雅,徐兵荣,肖箫,谢胜松,蒋羽.基于CRISPR/Cas系统的现场核酸检测技术[J].中国动物检疫,2022,39(3):55-60. |
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| 作者姓名： | 蒋静 赵文亮 李文越 陶大刚 潘文雅 徐兵荣 肖箫 谢胜松 蒋羽 |
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| 作者单位： | 上海海关动植物与食品检验检疫技术中心;上海海关机电产品检测技术中心;上海海关国际旅行卫生保健中心;华中农业大学;洋山海关 |
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| 基金项目： | 科技部重点研发计划项目（2018YFC1603600，2018YFC1603603）；上海海关科研项目（SHK007-2020） |
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| 摘 要： | 核酸检测技术在病原鉴定中发挥了至关重要的作用.传统核酸检测主要依赖聚合酶链式反应(polymerase chain reaction,PCR)技术,但该技术难以满足便携式现场核酸检测需求.近年来发明的CRISPR/Cas生物传感技术可实现高灵敏度、高特异性、快速准确的现场核酸检测.本文重点探讨了CRISPR/Cas生物...
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| 关 键 词： | CRISPR/Cas技术 核酸检测 现场可视化 |
An Review on a Technology for Field Detection of Nucleic Acids Based on CRISPR/Cas System |
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| Jiang Jing,Zhao Wenliang,Li Wenyue,Tao Dagang,Pan Weny,Xu Bingrong,Xiao Xiao,Xie Shengsong,Jiang Yu.An Review on a Technology for Field Detection of Nucleic Acids Based on CRISPR/Cas System[J].China Journal Of Animal Quarantine,2022,39(3):55-60. |
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| Authors: | Jiang Jing Zhao Wenliang Li Wenyue Tao Dagang Pan Weny Xu Bingrong Xiao Xiao Xie Shengsong Jiang Yu |
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| Institution: | (Technical Center for Animal Plant and Food Inspection and Quarantine of Shanghai Customs,Shanghai 200135,China;Technical Center for Mechanical and Electrical Product Inspection and Testing of Shanghai Customs Districte,Shanghai 201308,China;Shanghai International Travel Health Care Center,Shanghai 201206,China;Huazhong Agricultural University,Wuhan,Hubei 430070,China;Yangshan Customs,Shanghai 201308,China) |
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| Abstract: | The technology for detecting nucleic acids plays an important role in pathogen identification.Traditional detection of nucleic acids mainly depends on polymerase chain reaction(PCR)technology that is difficult to meet the needs for portable field detection of nucleic acids,which could be overcome by the CRISPR/Cas biosensor technology developed in recent years due to its high sensitivity,specificity,rapidity and accuracy.In the paper,the latest research progress of CRISPR/Cas biosensor technology was discussed,including its principle,advantages and disadvantages as well as application in field detection for nucleic acids.It was proved that the technology was very suitable for field detection of nucleic acids,which was believed to be widely used in the area in the future. |
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| Keywords: | CRISPR/Cas technology detection of nucleic acid field visualization |
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