| 上海市宠物猫杯状病毒、疱疹病毒及流感病毒的分离与鉴定 |
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| 引用本文： | 白艺兰,刘健,桂亚萍,夏炉明,龚国华,朱晓英,常晓静,陈伟锋,鞠厚斌,王建,赵洪进.上海市宠物猫杯状病毒、疱疹病毒及流感病毒的分离与鉴定[J].中国动物检疫,2022,39(3):13-18. |
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| 作者姓名： | 白艺兰 刘健 桂亚萍 夏炉明 龚国华 朱晓英 常晓静 陈伟锋 鞠厚斌 王建 赵洪进 |
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| 作者单位： | 上海市动物疫病预防控制中心 |
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| 基金项目： | 上海市科技兴农重点攻关项目（2021-02-08-00-12-F00772） |
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| 摘 要： | 为了解上海市猫上呼吸道疾病病例中猫杯状病毒(FCV)、猫疱疹病毒1型(FHV-1)和猫流感病毒(FIV)的感染比例及其遗传变化特点,对上海市冬季53份表现上呼吸道症状宠物猫的眼结膜、口咽和鼻黏膜拭子,进行FCV、FHV-1和FIV分离与鉴定,并对分离的病毒进行遗传进化分析.结果显示:53份样品中,FCV分离率为58.4...
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| 关 键 词： | 猫上呼吸道感染 猫杯状病毒 疱疹病毒 流感病毒 分离与鉴定 |
Isolation and Identification of Calicivirus,Herpesvirus and Influenza Virus in Pet Cats in Shanghai City |
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| Bai Yilan,Liu Jian,Gui Yaping,Xia Luming,Gong Guohu,Zhu Xiaoying,Chang Xiaojin,Chen Weifeng,Ju Houbin,Wang Jian,Zhao Hongjin.Isolation and Identification of Calicivirus,Herpesvirus and Influenza Virus in Pet Cats in Shanghai City[J].China Journal Of Animal Quarantine,2022,39(3):13-18. |
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| Authors: | Bai Yilan Liu Jian Gui Yaping Xia Luming Gong Guohu Zhu Xiaoying Chang Xiaojin Chen Weifeng Ju Houbin Wang Jian Zhao Hongjin |
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| Institution: | (Shanghai Animal Disease Prevention and Control Center,Shanghai 201103,China) |
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| Abstract: | In order to investigate the infection proportion and genetic changes of feline calicivirus(FCV),feline herpesvirus type 1(FHV-1)and feline influenza virus(FIV)in cats infected with upper re spiratory tract diseases(URTDs)in Shanghai City,53 conjunctival,oral and nasal mucosa swabs collected from pet cats showing the symptoms of URTDs in winters were detected to isolate and identify the above viruses,followed by genetic evolution analysis for the isolated viruses.The results showed that,for the 53 samples,the isolation rate was 58.49%(31/53)for FCV and 3.77%(2/53)for FHV-1;and no FIV was isolated.For FCV positive samples,the isolation rate was 46.67%(14/30)from the cats in pet hospitals,and up to 73.91%(17/23)from those in cat houses.The nucleotide homology of VP1 gene of 31 FCV isolates was lower,which was 72.9%-86.7%with the reference sequence and 75.3%-99.8%between each other.In conclusion,FCV was a major pathogen for URTDs in cats,with complex evolutionary relationship of virus and extensive sources,which should be further investigated and monitored.In the paper,a basis was thereby provided for future prevention,control and treatment of URTDs in cats,and a sound foundation for development of diagnostic reagents and vaccines against such diseases. |
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| Keywords: | URTDs FCV FHV-1 FIV isolation and identification |
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