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| 锯缘青蟹血清凝集素的特性和盐析提取研究 | |
| 引用本文： | 郝珂,钱冬,刘问,潘清清.锯缘青蟹血清凝集素的特性和盐析提取研究[J].淡水渔业,2008,38(4). |
| 作者姓名： | 郝珂 钱冬 刘问 潘清清 |
| 作者单位： | 1. 宁波大学生命科学与生物工程学院,浙江,宁波,315211;浙江省淡水水产研究所,浙江,湖州,313001 2. 浙江省淡水水产研究所,浙江,湖州,313001 3. 浙江省淡水水产研究所,浙江,湖州,313001;华中农业大学,武汉,430070 |
| 摘 要： | 对锯缘青蟹(Scylla serrate)血清凝集素的凝集特性进行了研究。结果显示:锯缘青蟹血清凝集素对人、兔、鼠、鸽子、中华鳖、鲫红细胞均有凝集作用,其中对小鼠红细胞凝集活性最强,血凝活性可达29;凝集素活性最适pH 6.0~7.4;盐度对凝集活力有较大影响,NaC l浓度在0.4~0.5 mol/L时基本失活;未纯化血清凝集素对Ca2+、Mg2+未表现出依赖性;凝集素活性能被N-乙酰葡萄糖胺特异性抑制。对青蟹血清凝集素进行硫酸铵分级沉淀分离后,发现凝集素活性主要分布在25%饱和度硫酸铵沉淀区,该组分凝集比活比血清高27.44倍,SDS-PAGE结果表明主要蛋白带型为79 ku、75 ku、72 ku。 |
| 关 键 词： | 锯缘青蟹(Scyllaserrate) 血清凝集素 凝集活性 盐析提取 |
Studies on Characteristics and Salting-out Extraction of Hemolymph Lectin from Mud Crab,Scylla serrate |
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| HAO Ke,QIAN Dong,LIU Wen,PAN Qing-qing.Studies on Characteristics and Salting-out Extraction of Hemolymph Lectin from Mud Crab,Scylla serrate[J].Freshwater Fisheries,2008,38(4). | |
| Authors: | HAO Ke QIAN Dong LIU Wen PAN Qing-qing |
| Abstract: | Agglutination and main characteristics of hemolymph lectin from mud crab,Scylla serrate were studied.The results showed that the hemolymph lectin from mud crab could agglutinate the erythrocytes of human,rabbit,mouse,pigeon chinese soft-shelled turtle and crucian carp,with the highest agglutinating activity(29) for mouse erythrocyte.The optimal pH of agglutination for hemolymph lectin was 6.0~7.4,and agglutination could be inhibited by NaCl at a concentration of 0.4~0.5 mol/L.No dependence on Ca2+ and Mg2+ was found when serum was applied for lectin agglutination.The mud crab lectin agglutination could be inhibited by N-acetylglucosamine.When ammonium sulfate was used for fractionation of lectin from mud crab serum,highest agglutinating activity was found in 25% saturation precipitated fraction,27.44 times higher than original serum,with three main protein bands sized of 79 ku、75 ku、72 ku by SDS-PAGE. |
| Keywords: | Scylla serrate hemolymph lectin agglutination salting-out extraction |
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