| 白粉病菌侵染诱导的大麦叶肉细胞变化的超微结构与细胞化学 |
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| 引用本文： | 赵淑芳,胡东维.白粉病菌侵染诱导的大麦叶肉细胞变化的超微结构与细胞化学[J].植物病理学报,2003,33(5):444-448. |
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| 作者姓名： | 赵淑芳 胡东维 |
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| 作者单位： | 浙江大学生物技术研究所, 杭州 310029 |
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| 基金项目： | 国家自然科学基金资助项目(30270859) |
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| 摘 要： | 从超微结构和细胞化学角度对大麦白粉病菌侵染诱导的寄主叶肉细胞变化进行了研究。结果发现,病原菌接种后12 h,叶肉细胞光合作用的标志性酶RCA和Rubisco含量发生迅速的变化,感病大麦(Ingrid)中RCA和Rubisco含量急剧减少,而抗病大麦(mlo-3)中则趋势相反。随后,不论在感病或抗病大麦中这2种蛋白的含量一直下降,但感病品种中一直维持相对较高水平。超微结构观察表明,病原菌侵染能够诱导侵染点附近叶肉细胞中叶绿体的增殖,新叶绿体的不断产生,从而延缓了叶肉细胞的衰老和死亡,增加了光合产物的形成。以上结果说明,叶肉细胞可能从养分供给上影响到了表皮细胞中病原菌吸器的发育。
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| 关 键 词： | 大麦 白粉病菌 细胞化学 超微结构 抗病性 |
| 文章编号： | 0412-0914(2003)05-0444-05 |
| 修稿时间： | 2003年3月24日 |
Ultrastructure and cytochemistry of barley mesophyll attacked by Blumeria graminis f. sp. hordei |
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| ZHAO Shu-fang,HU Dong-wei.Ultrastructure and cytochemistry of barley mesophyll attacked by Blumeria graminis f. sp. hordei[J].Acta Phytopathologica Sinica,2003,33(5):444-448. |
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| Authors: | ZHAO Shu-fang HU Dong-wei |
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| Institution: | Biotechnology Institute, Zhejiang University, Hangzhou 310029, China |
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| Abstract: | Ultrastructure and cytochemistry in barley mesophyll infected with Blumeria graminis f. sp. hordei were investigated. The results showed that the contents of Rubisco and RCA changed rapidly at 12 h after inoculation. The contents in the susceptible cultivar, Ingrid, decreased rapidly, but that in the resistance cultivar, mlo-3, increased. Subsequently, the contents of Rubisco and RCA decreased gradually both in Ingrid and mlo-3, while the contents in Ingrid always kept higher than that in mlo-3. Ultrastructure observation released that the senescence of chloroplast was expedited after inoculation, and its regeneration in the mesophyll next to the attacked epidermis was the main reason for the formation of green island, which can supply the pathogen more products of photosynthesis. All these results indicated that the development of the haustoria was possibly affected by mesophyll in aspect of nutrition supply. |
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| Keywords: | barley Blumeria graminis f sp hordei cytochemistry ultrastructure resistance |
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