| 棉花油菜素类固醇合成酶基因GhDWF1的克隆和表达分析 |
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| 引用本文： | 罗明,周建平,肖月华,李先碧,侯磊,肖忠意,裴炎.棉花油菜素类固醇合成酶基因GhDWF1的克隆和表达分析[J].中国农业科学,2007,40(7):1337-1344. |
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| 作者姓名： | 罗明 周建平 肖月华 李先碧 侯磊 肖忠意 裴炎 |
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| 作者单位： | 西南大学生物技术中心,重庆400716 |
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| 基金项目： | 国家自然科学基金资助项目（30530490、30671258和30370904）、国家基础研究和发展计划（2004CB117300）、国家高技术研究发展计划（2006AA102121） |
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| 摘 要： | 【目的】研究油菜素类固醇物质（BRs）在棉花纤维生长发育过程中的作用。【方法】通过棉花EST序列的筛选和整合，从陆地棉徐州142纤维中克隆了一个BRs合成酶基因GhDWF1。【结果】其cDNA序列全长1 849 bp，包含一个1 692 bp的开放阅读框，推导编码563个氨基酸，与水稻、玉米、豌豆、番茄和拟南芥的BRs合成酶DWARF1/DIMINUTO有较高的同源性，具有该类蛋白的典型结构。GhDWF1基因在开花当天的胚珠和纤维中表达最强，在根、幼茎和纤维发育的各个时期表达较高。与徐州142野生型相比，无绒无絮突变体胚珠中表达量较低。【结论】暗示GhDWF1基因的表达与棉花纤维的生长发育有密切关系。
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| 关 键 词： | 棉花 DWARF1基因 纤维 油菜素类固醇 植物固醇 |
| 收稿时间： | 2006-5-22 |
| 修稿时间： | 2006-05-192006-09-25 |
Cloning and Expression Analysis of A Brassinosteroid Biosynthetic Enzyme Gene,GhDWF1,from Cotton (Gossypium hirsuturm L.) |
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| LUO Ming, ZHOU Jian-ping, XIAO Yue-hua, LI Xian-bi, HOU Lei, XIAO Zhong-yi, PEI Yan.Cloning and Expression Analysis of A Brassinosteroid Biosynthetic Enzyme Gene,GhDWF1,from Cotton (Gossypium hirsuturm L.)[J].Scientia Agricultura Sinica,2007,40(7):1337-1344. |
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| Authors: | LUO Ming ZHOU Jian-ping XIAO Yue-hua LI Xian-bi HOU Lei XIAO Zhong-yi PEI Yan |
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| Institution: | Center of Biotechnology, Southwest University, Chongqing 400716 |
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| Abstract: | Brassinosteroids (BRs) are an important class of plant steroidal hormones that are essential in a wide variety of physiological processes, including cell division and elongation, vascular differentiation, reproductive development, senescence, biotic and abiotic tolerance. To determine the effects of BRs on development of cotton fibers, we have cloned a key gene (GhDWF1) involved in the upstream biosynthetic pathway of BRs from developing fibers of upland cotton (Gossypium hirsutum L.) cv. XuZhou142. The full length of the cloned cDNA is 1849bp, including a 37bp 5’-untranslated region, an ORF of 1692bp and a 120bp 3’-untranslated region. The cDNA encodes a polypeptide of 563 amino acid residues with a predicted molecular mass of 65 KD. The deduced amino acid sequence has high homology with the BR biosynthetic enzyme, DWARF1/DIMINUTO, from rice, maize, pea, tomato and Arabidopsis. Furthermore, the typical conserved structure, such as the transmembrane domain, the FAD-dependent oxidase domain and FAD-binding site, are present in GhDWF1 protein. Southern blot indicated the GhDWF1 gene has a single copy in upland cotton genome. RT-PCR analysis revealed that the highest level of GhDWF1 expression was detected in 0DPA ovule (with fibers) while the lowest level was observed in cotyledon. The GhDWF1 gene expressed highly in roots, young stems and fibers. Moreover, the expression level was higher in ovules (with fibers) of wildtype (XuZhou142) than in ovules of fuzzless-lintless mutant at the same developmental stages (0DPA and 4DPA). Results suggest that the GhDWF1 gene plays a crucial role in fiber development, especially, in the initiation and the secondary cell wall deposition of fiber cells. |
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| Keywords: | Cotton DWARF1 gene Fiber Brassinosteroids Phytosterol |
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