A novel cold-inducible promoter,P<Emphasis Type="Italic">ThCAP</Emphasis> from <Emphasis Type="Italic">Tamarix hispida</Emphasis>, confers cold tolerance in transgenic <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> |
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| Authors: | Xiaohong Guo Yandong Lv Hongyu Li Nan Fu Guiping Zheng Lihua Liu Yuhua Li |
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| Institution: | 1.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,People’s Republic of China;2.Key Laboratory of Modern Agricultural Cultivation and Crop Germplasm Improvement of Heilongjiang Province Agronomy College,Heilongjiang Bayi Agricultural University,Daqing,People’s Republic of China;3.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,People’s Republic of China;4.College of Life Sciences, Daqing Bio-tech Research Institute,Northeast Forestry University,Harbin,People’s Republic of China |
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| Abstract: | Our previous studies have revealed that the ThCAP gene plays a vital role in transgenic Populus (P. davidiana × P. bolleana) in response to cold stress. However, the regulatory mechanism of ThCAP gene expression has been unclear. In this study, the 5′ flanking region of the ThCAP promoter (PThCAP) was cloned using a genome-walking method. By analyzing cis-acting regulatory elements of PThCAP, a DRE motif and MYC and MYB elements were found to be located in the promoter. To identify the regulatory elements that control the expression of the ThCAP gene promoter, a series of deletion derivatives of PThCAP, P1–P5, from the translation start code (?1538, ?1190, ?900, ?718 and ?375 bp), were fused to the GUS reporter gene, and then each deletion was stably introduced into Arabidopsis thaliana plants. Deletion analysis of the promoter suggested that only the P2 fragment had strong GUS expression in leaves and roots of A. thaliana exposed to low temperature stress. These results suggest that this 290-bp region (?1190 to ?900 bp), as an important part in PThCAP, was associated with cold tolerance of A. thaliana. Our results provide evidence for the regulatory mechanism of ThCAP gene involved in the response to cold stress, and that the gene is promising candidate gene for genetic improvement of crops. |
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