| “胰岛素-转铁蛋白-硒钠”对山羊卵母细胞体外成熟及胚胎发育的影响(英文) |
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| 引用本文： | 刘凤军,张玉玲,杨自军,王国华,张涌.“胰岛素-转铁蛋白-硒钠”对山羊卵母细胞体外成熟及胚胎发育的影响(英文)[J].农业科学与技术,2008,9(3):107-110. |
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| 作者姓名： | 刘凤军 张玉玲 杨自军 王国华 张涌 |
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| 作者单位： | 河南科技大学动物科技学院;西北农林科技大学生物工程研究所 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，河南科技大学博士基金 |
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| 摘 要： | Objective] The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and to explore serum-free culture system in vitro.Method] At present,the conventional solutions of oocyte maturation and embryo development in vitro were always added into 1% ITS(Insulin-transferrin-selenium) or using 1% ITS to replace FBS in 2 kinds culture solutions for conducting in vitro cultures of goat oocyte and parthenogenetic embryo.The influences of ITS on their developments were detected.Result] ITS in maturation liquid of oocytes could not increase oocytes maturation rate but significantly increased blastocyst rate (58.06% vs. 48.19%)of parthenogenetic embryo.If FBS in maturation liquid of oocytes was replaced by ITS, the maturation rate, cleavage rate and blastocyst rate were basically unchanged.Adding ITS into embryo medium could increase blastocyst rate (68.30% vs. 56.82%)of parthenogenetic embryo of goat.If FBS in embryo medium was replaced by ITS,the cleavage rate didn’t change basically,while the blastocyst rate in ITS was obviously lower than that in FBS group(42.33% vs.56.82%).Conclusion] ITS could promote maturation of oocyte in vitro and early embryonic development, in addition,ITS could replace serum in maturation medium of oocyte as serum-free culture system for conducting relevant researches.
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| 关 键 词： | Insulin-transferrin-selenium Free-serum culture Oocyte Embryo Parthenogenesis Goat |
Effect of Insulin-Transferrin-Selenium on Goat Oocytes Maturation and Embryo Development |
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| LIU Feng-jun,ZHANG Yu-ling,YANG Zi-jun,WANG Guo-hua,ZHANG Yong.Effect of Insulin-Transferrin-Selenium on Goat Oocytes Maturation and Embryo Development[J].Agricultural Science ＆ Technology,2008,9(3):107-110. |
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| Authors: | LIU Feng-jun ZHANG Yu-ling YANG Zi-jun WANG Guo-hua ZHANG Yong |
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| Institution: | 1. College of Animal Science and Technology,Henan University of Science and Technology, Luoyang 471003;2. Research Institute of Biotechnology,Northwest A & F University, Yangling 712100 |
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| Abstract: | Objective] The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and to explore serum-free culture system in vitro.Method] At present,the conventional solutions of oocyte maturation and embryo development in vitro were always added into 1% ITS(Insulin-transferrin-selenium) or using 1% ITS to replace FBS in 2 kinds culture solutions for conducting in vitro cultures of goat oocyte and parthenogenetic embryo.The influences of ITS on their developments were detected.Result] ITS in maturation liquid of oocytes could not increase oocytes maturation rate but significantly increased blastocyst rate (58.06% vs. 48.19%)of parthenogenetic embryo.If FBS in maturation liquid of oocytes was replaced by ITS, the maturation rate, cleavage rate and blastocyst rate were basically unchanged.Adding ITS into embryo medium could increase blastocyst rate (68.30% vs. 56.82%)of parthenogenetic embryo of goat.If FBS in embryo medium was replaced by ITS,the cleavage rate didn't change basically,while the blastocyst rate in ITS was obviously lower than that in FBS group(42.33% vs.56.82%).Conclusion] ITS could promote maturation of oocyte in vitro and early embryonic development, in addition,ITS could replace serum in maturation medium of oocyte as serum-free culture system for conducting relevant researches. |
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| Keywords: | Insulin-transferrin-selenium Free-serum culture Oocyte Embryo Parthenogenesis Goat |
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