| 近缘毛壳Chaetomium subaffine LB-1抑菌物质存在部位及提取方法 |
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| 引用本文： | 刘彩云,高秀清,赵静.近缘毛壳Chaetomium subaffine LB-1抑菌物质存在部位及提取方法[J].华南农业大学学报,2023,44(2):280-286. |
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| 作者姓名： | 刘彩云 高秀清 赵静 |
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| 作者单位： | 潍坊学院 种子与设施农业工程学院, 山东 潍坊 261061 |
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| 基金项目： | 山东省自然科学基金(ZR2021MC144) |
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| 摘 要： | 【目的】对近缘毛壳Chaetomium subaffine菌株LB-1产生抑菌物质的部位及提取方法进行研究，为开发生防菌株LB-1防治植物病害奠定基础。【方法】以番茄灰霉病菌Botrytis cinerea和玉米大斑病菌Exserohilum turcicum为指示菌，采用平板密封培养法检测菌株LB-1是否产生挥发性抑菌物质；采用菌体超声破碎和液态培养的方式检测菌株LB-1产生的非挥发性抑菌物质存在的部位；采用含毒培养基法和滤纸片法检测菌株LB-1培养液的硫酸铵沉淀、盐酸沉淀和有机溶剂萃取物的抑菌效果，以确定菌株LB-1培养液中抑菌物质的提取方法。【结果】菌株LB-1与2种供试病原菌密封共培养时，对病原菌的生长没有明显影响，表明菌株LB-1不能产生挥发性抑菌物质。菌株LB-1菌体胞内提取物的抑菌活性与对照无差异，但其培养液对B. cinerea和E. turcicum的生长有较强的抑制作用，表明菌株LB-1产生的抑菌物质存在于菌体细胞外。菌株LB-1培养液的硫酸铵沉淀和盐酸沉淀对2种供试植物病原真菌的生长均无抑制效果，但有机溶剂萃取可获得菌株LB-1培养液中的抑菌物质，其中正丁醇萃取物...
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| 关 键 词： | 近缘毛壳 抑菌物质 培养液 正丁醇粗提物 |
| 收稿时间： | 2022/4/12 0:00:00 |
Existing position and extraction of antifungal substance produced by Chaetomium subaffine LB-1 |
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| LIU Caiyun,GAO Xiuqing,ZHAO Jing.Existing position and extraction of antifungal substance produced by Chaetomium subaffine LB-1[J].Journal of South China Agricultural University,2023,44(2):280-286. |
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| Authors: | LIU Caiyun GAO Xiuqing ZHAO Jing |
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| Institution: | College of Seed and Facility Agricultural Engineering, Weifang Universtiy, Weifang 261061, China |
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| Abstract: | Objective The existing position and extraction method of antifungal substance produced by Chaetomium subaffine strain LB-1 were studied in order to lay a foundation for the development of the strain LB-1 to control plant diseases.Method With Botrytis cinerea and Exserohilum turcicum as test plant pathogens, the sealed plate assay was used to detect whether the strain LB-1 could produce volatile antifungal substance. The hyphae ultrasonic breaking and liquid culture methods were used to detect the existing position of nonvolatile antifungal substance produced by the strain LB-1. The extraction method of antifungal substance was determined by detecting the inhibitory effects of ammonium sulfate precipitation, hydrochloric acid precipitation and organic solvents extracts of strain LB-1 culture broth via poison plate assay and filter paper disc assay.Result The strain LB-1 did not have obvious inhibitory effect on the growth of the two test plant pathogens when being co-cultured in a sealed plate with each plant pathogen, indicating that strain LB-1 could not produce volatile antifungal substance. The antifungal activity of the intracellular extract of the strain LB-1 was not different from that of the control, but its culture broth had a strong inhibitory effect on B. cinerea and E. turcicum, indicating that the antifungal substances produced by the strain LB-1 existed outside the mycelium. Neither ammonium sulfate precipitate nor hydrochloric acid precipitate of strain LB-1 culture broth showed inhibitory effect on B. cinerea and E. turcicum, but the organic solvent extract of strain LB-1 culture broth showed antifungal effect, and the inhibition rate of n-butanol extract was the highest. When the concentration was 0.1 mg/mL, the inhibition rates against B. cinerea and E. turcicum growth were 59.80% and 58.37% respectively.Conclusion The strain LB-1 inhibited the growth of plant pathogenic fungi by producing extracellular nonvolatile antifungal substances, and the antifungal substance in the culture broth can be extracted by n-butanol. |
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| Keywords: | Chaetomium subaffine Antifungal substance Culture broth Crude extract of n-butanol |
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