| 原生质体融合构建水稻病害生防多功能工程菌株 |
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| 引用本文： | 陈海荣,刘前刚,高书锋,魏小武,程安,黄军.原生质体融合构建水稻病害生防多功能工程菌株[J].湖南农业科学,2010(1):13-15. |
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| 作者姓名： | 陈海荣 刘前刚 高书锋 魏小武 程安 黄军 |
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| 作者单位： | 湖南省微生物研究所,湖南长沙,410009 |
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| 基金项目： | 湖南省农业厅资助项目 |
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| 摘 要： | 选取具有杀虫效果的苏云金芽孢杆菌（Bt）和抗病效果的枯草芽孢杆菌（SD）进行原生质体融合。通过对融合条件的摸索，得出Bt的最佳培养时间为5h，最佳酶解浓度为0．8mg／mL，最佳酶解时间为70min；SD最佳培养时间为5h，最佳酶解浓度为0．6mg／mL，最佳酶解时间为40min。通过检测，试验得出的融合子具备双亲的功效，
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| 关 键 词： | 原生质体 融合 Bt SD 融合子 |
Constructing Multi-function Engineering Bacteria of Rice by Protoplast Fusion |
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| CHEN Hai-rong,LIU Qian-gang,GAO Shu-feng,WEI Xiao-wu,CHENG An,HUANG Jun.Constructing Multi-function Engineering Bacteria of Rice by Protoplast Fusion[J].Hunan Agricultural Sciences,2010(1):13-15. |
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| Authors: | CHEN Hai-rong LIU Qian-gang GAO Shu-feng WEI Xiao-wu CHENG An HUANG Jun |
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| Abstract: | The insecticidal Bacillus thuringieusis (Bt) and disease-resistant Bacillus subtilis (SD) were selected for protoplast fusion. Through the exploration for fusion condition, the optimum cultural conditions for Bt were concluded as follows: the optimum cultural time was 5 h, the optimum concentration of enzymolysis was 0.8 mg/ml, and the opfimmtimeof enzymolysis was 70 mins. The optimum cultural conditions for SD were concluded as follows: the optimum cultural time was 5 h, the optimum concentration of enzymolysis was 0.6 mg/ml, and the optimum time of enzymolysis was 40 mins. Through the test, the results indicated that the fusant, which got from the experiment, have the efficacies of both parents. |
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| Keywords: | Bt SD protoplast fusion Bt SD fusant |
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