Differential effects of tomato (Lycopersicon esculentum mill) matrix on the volatility of important aroma compounds |
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| Authors: | Bezman Yair Mayer Florian Takeoka Gary R Buttery Ron G Ben-Oliel Gad Rabinowitch Haim D Naim Michael |
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| Institution: | Institutes of Biochemistry, Food Science and Nutrition and of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel. |
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| Abstract: | Significant tomato matrix effects on the volatility of certain fresh tomato odorants were found. The concentrations of odorants such as (E,E)-2,4-decadienal, beta-damascenone, and beta-ionone, in crushed fresh tomato fruit obtained by solid-phase microextraction (SPME), resulting from a tomato matrix calibration curve were 5.5-, 2-, and 12-fold higher, respectively, than those calculated by calibration based on buffer solutions. Static headspace analyses indicated that, in most cases, the tomato matrix significantly retains the odorants relative to the buffer solution. Thus, the concentration of odorants in the headspace of tomato is lower than expected compared to a simple matrix such as buffer. CaCl(2), although needed in crushed fruit tissue to block enzymatic activity, was found to interact specifically with 2-isobutylthiazole, reducing its content in the headspace by at least 6-fold. If a matrix effect is found, analysis of the odorant molecule contents in the headspace rather than in the food is recommended in order to better evaluate their access to the olfactory receptors. |
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