| 盾叶薯蓣原生质体分离 |
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| 引用本文： | 刘艳丽,姚家玲.盾叶薯蓣原生质体分离[J].华中农业大学学报,2008,27(3). |
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| 作者姓名： | 刘艳丽 姚家玲 |
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| 作者单位： | 1. 武汉科技大学中南分校生命科学学院,武汉,430223;华中农业大学生命科学技术学院,武汉,430070
2. 华中农业大学生命科学技术学院,武汉,430070 |
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| 摘 要： | 以盾叶薯蓣叶片、种子愈伤为材料,探讨了不同的酶浓度、酶解时间及渗透压对原生质体分离的影响.结果表明:生长5～10d的叶片置于0.6%纤维素酶、0.5%果胶酶、0.9mol/L甘露醇的酶液中、黑暗处理21h,其原生质体产量和活力最高;悬浮培养5～10d的愈伤组织在2.0%纤维素酶、1.0%果胶酶、0.7mol/L甘露醇的混合酶液中黑暗处理6～8h原生质体产量和活力最高.
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| 关 键 词： | 盾叶薯蓣 原生质体 分离 |
Protoplast Isolation in Dioscorea zingibernsis Wright |
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| LIU Yan-li,YAO Jia-ling.Protoplast Isolation in Dioscorea zingibernsis Wright[J].Journal of Huazhong Agricultural University,2008,27(3). |
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| Authors: | LIU Yan-li YAO Jia-ling |
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| Abstract: | Young leaves,and calli were excised as material,and the effect of different enzyme combination,time of enzymolysis,osmotic pressure on protoplast isolation were studied.The results are as follow:Leaves which have grown for 5~10 days are cut up,then were put into enzyme which consists of 0.6% cellulase,0.5% pectinase and 0.9 mol/L mannitol and are sustaining for 21 hours in dark condition,its protoplast yield and activity is the highest;Calli which have suspended for 5 days are put into enzyme including 2.0%... |
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| Keywords: | Dioscorea zingiberensis Wright protoplast isolation |
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