| 三个棉花雄性不育株花药发育的过程 |
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| 作者单位： | 南京农业大学农学院, 作物遗传与种质创新国家重点实验室，江苏 南京 210095 |
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| 基金项目： | 国家自然科学基金 (10475041) |
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| 摘 要： | 利用20Gy的60Co射线对陆地棉品种苏棉22号的花粉进行辐照处理，处理后的花粉给雌蕊授粉，并收获种子。从M1群体中选取3个雄性不育株，采用石蜡切片技术对花药发育过程进行研究。通过与对照比较，3个雄性不育株各个时期的败育特征有一定差异，但它们的主要败育特征是一致的：从小孢子母细胞时期至小孢子发育阶段都有败育，小孢子母细胞、绒毡层和中层细胞以及花药的形态都受到不同程度的影响，包括小孢子母细胞有微核及双核现象、减数分裂阶段小孢子母细胞的细胞质膨胀、绒毡层及中层细胞异常等。
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| 关 键 词： | 辐射诱变 雄性不育 花药 陆地棉 |
| 收稿时间： | 2009-03-02 |
The Anther Development Research on Three Male-sterile Lines Cotton |
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| Institution: | Agronomy College, National Key Laboratory of Crop Genetics ＆ Germplasm Enhancement Agricultural University, Nanjing Agricultural University, Nanjing, Jiangsu 210095 |
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| Abstract: | Pollens of Sumian22 were irradiated by 60Coγ- rays at a dose of 20Gy，then fertilized to pistil and harvested seed. Three male-sterile lines were selected from M1 plants, their anther observed by paraffin slice technique. Although there were some different characteristics during the abortion anther development, the abortion was consistent：the abortion stage from the development periods of pollen mother cells to microspore， pollen mother cells, tapetum, middle layer cells and the shape of anther were affected, the results contained micronucleus and double nucleus, cytoplasm expansion during the period of meiosis, tapetum and middle layer cells were abnormal, and so on were abnormal. |
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| Keywords: | irradiation-induced mutation male-sterile anther upland cotton |
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